Typing tool
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Typing tool
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Complete norovirus genomes
| KJ685409 | GII.4 New Orleans | |
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| GII.P4 New Orleans |
ORF1: 1..5041
ORF2: 5022..6644
ORF3: 6644..7450
LOCUS KJ685409 7450 bp ss-RNA linear VRL 28-JAN-2015
DEFINITION Norovirus Hu/GII/BG1C0241-32/2011/BGD, partial genome.
ACCESSION KJ685409
VERSION KJ685409.1
DBLINK BioProject: PRJNA242747
KEYWORDS .
SOURCE Norovirus Hu/GII/BG1C0241-32/2011/BGD
ORGANISM Norovirus Hu/GII/BG1C0241-32/2011/BGD
Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
Picornavirales; Caliciviridae; Norovirus; Norwalk virus.
REFERENCE 1 (bases 1 to 7450)
AUTHORS Das,S.R., Halpin,R.A., Mohan,M., Fedorova,N., Tsitrin,T., Puri,V.,
Stockwell,T., Amedeo,P., Bishop,B., Gupta,N., Hoover,J., Katzel,D.,
Schobel,S., Shrivastava,S., Ahmed,T., Haque,R., Knobler,S.,
Miller,M., Wentworth,D.E. and Nelson,M.
TITLE Direct Submission
JOURNAL Submitted (03-APR-2014) J. Craig Venter Institute, 9704 Medical
Center Drive, Rockville, MD 20850, USA
COMMENT This work was supported by the National Institute of Allergy and
Infectious Diseases (NIAID), Genome Sequencing Centers for
Infectious Diseases (GSCID) program.
The genome sequence was generated using overlapping PCR amplicons
spanning the genome. The amplicons were pooled by sample and then
barcoded and sequenced using Next Generation Sequencing platforms.
The consensus sequences of the internal PCR primer hybridization
sites were manually verified using reads from amplicons that
spanned across the sites.
##Genome-Assembly-Data-START##
Current Finishing Status :: Finished
Assembly Method :: clc_ref_assemble_long v. 3.22.55705
Genome Coverage :: 185.3x
Sequencing Technology :: Ion Torrent
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7450
/organism="Norovirus Hu/GII/BG1C0241-32/2011/BGD"
/mol_type="genomic RNA"
/strain="Hu/GII/BG1C0241-32/2011/BGD"
/host="Homo sapiens; sex: F; age: 204D"
/db_xref="taxon:1486688"
/country="Bangladesh: Dhaka"
/collection_date="13-Jul-2011"
/PCR_primers="fwd_name: Ampl_1_Forward, fwd_seq:
gtgaatgawgatggcgt, rev_name: Ampl_1_Reverse, rev_seq:
ttggttgagagyttyctg"
/PCR_primers="fwd_name: Ampl_2_Forward, fwd_seq:
ccgcaaaatcttcaagtg, rev_name: Ampl_2_Reverse, rev_seq:
cwacaggtcttggtctgctrga"
/PCR_primers="fwd_name: Ampl_3_Forward, fwd_seq:
tcaaccaartctgcttcacctg, rev_name: Ampl_3_Reverse, rev_seq:
ratcctttgccggatcttgg"
/PCR_primers="fwd_name: Ampl_4_Forward, fwd_seq:
ggcaagaagcacacagcctt, rev_name: Ampl_4_Reverse, rev_seq:
crrctctytgttgtgttgaatccc"
/PCR_primers="fwd_name: Ampl_5_Forward, fwd_seq:
tggyaagatcaagaagaggc, rev_name: Ampl_5_Reverse, rev_seq:
acaacaaargcacygctrgg"
/PCR_primers="fwd_name: Ampl_6_Forward, fwd_seq:
gagrccrtccccygattttg, rev_name: Ampl_6_Reverse, rev_seq:
atcaatyttgtcttttcaca"
/note="genotype: GII"
gene <1..5041
/gene="POL"
/locus_tag="DC54_49757gpPOL"
CDS <1..5041
/gene="POL"
/locus_tag="DC54_49757gpPOL"
/note="genome polyprotein"
/codon_start=2
/product="nonstructural polyprotein"
/protein_id="AHX22034.1"
/translation="AKSSSDGVLSSMAVTFKRALGARPKQPPPREKPQRPPRPPTPEL
VKNIPPPPPNGEDEIVVSYSVKDGVSGLPDLSTVRQPEESNTAFSVPPLNQRENRDAK
EPLTGTILEMWDGEIYHYGLYVERGLVLGVHKPPAAISLAKVELAPLSLYWRPVYTPQ
YLISPDTLKKLSGETFPYTAFDNNCYAFCCWVLDLNDSWLSRRMIQRTTGFFRPYQDW
NRKPLPTMDDSKIKKVANIFLCALSSLFTRPIKDIIGKIRPLNILNILASCDWTFAGI
VESLILLAELFGVFWTPPDVSAMIAPLLGDYELQGPEDLAVELVPVVMGGIGLVLGFT
KEKIGKMLSSAASTLRACKDLGAYGLEILKLVMKWFFPKKEEANELAIVRSIEDAVLD
LEAIENNHMTTLLKDKDSLATYMRTLDLEEEKARKLSTKSASPDIVGTINALLARIAA
ARSLVHRAKEELSSRPRPVVLMISGRPGIGKTHLAREVAKRIAASLTGDQRVGLIPRN
GVDHWDAYKGERVVLWDDYGMSNPIHDALRLQELADTCPLTLNCDRIENKGKVFDSDV
IIITTNLANPAPLDYVNFEACSRRIDFLVYAEAPEVEKAKRDFPGQPDMWKNAFSSDF
SHIKLALAPQGGFDKNGNTPHGKGVMKTLTTGSLIARASGLLHERLDEFELQGPALTT
FNFDRNKVLAFRQLAAENKYGLVDTMRVGKQLKDVRTMPELKQALKNVSIKKCQIVYS
GCTYMLESDGKGNVKVDRIQSAAVQTNNELAGALHHLRCARIRYYVKCVQEALYSIIQ
IAGAAFVTTRIAKRMNIQDLWSKPQVENTEETTSKDGCPKPKDDEEFVISSDDIKTEG
KKGKNKTGRGKKHTAFSSKGLSDEEYDEYKRIREERNGKYSIEEYLQDRDKYYEEVAI
ARATEEDFCEEEEAKIRQRIFRPTRKQRKEERVSLGLVTGSEIRKRNPDDFKPKGKLW
ADDDRSVDYNEKLSFEAPPSIWSRIVNFGSGWGFWVSPSLFITSTHVIPQGAKEFFGV
PIKQIQVHKSGEFCRLRFPKPIRTDVTGMILEEGAPEGTVVTLLIKRSTGELMPLAAR
MGTHATMKIQGRTVGGQMGMLLTGSNAKSMDLGTTPGDCGCPYIYKRGNDYVVIGVHT
AAARGGNTVICATQGSEGEATLEGGDNKGTYCGAPILGPGSAPTLSTKTKFWRSSTAS
LPPGTYEPAYLGGKDPRVKGGPSLQQVMREQLKPFTEPRGKPPKPSVLEAAKKTIINV
LEQTIDPPEKWSFAQACASLDKTTSSGHPHHMRKNDCWNGESFTGKLADQASKANLMF
EEGKNMTPVYTAALKDELVKTDKIYGKIKKRLLWGSDLATMIRCARAFGGLMDELKAH
CVTLPIRVGMNMNEDGPIIFERHSRYTYHYDADYSRWDSTQQRAVLAAALEIMVKFSP
EPHLAQVVAEDLLSPSVVDVGDFTISINEGLPSGVPCTSQWNSIAHWLLTLCALSEVT
NLSPDTIQANSLFSFYGDDEIVSTDIKLDPEKLTAKLREYGLKPTRPDKTEGPLVISE
DLNGLTFLRRTVTRDPAGWFGKLEQSSILRQMYWTRGPNHGDPSETMIPHSQRPIQLM
SLLGEAALHGPAFYSKISKLVIAELKEGGMDFYVPRQEPMFRWMRFSDLSTWEGDRNL
APSFVNEDGVE"
mat_peptide <1..931
/gene="POL"
/locus_tag="DC54_49757gpPOL"
/product="putative protein p48"
mat_peptide 932..2029
/gene="POL"
/locus_tag="DC54_49757gpPOL"
/product="NTPase"
/note="p41"
mat_peptide 2030..2566
/gene="POL"
/locus_tag="DC54_49757gpPOL"
/product="protein p22"
mat_peptide 2567..2965
/gene="POL"
/locus_tag="DC54_49757gpPOL"
/product="viral genome-linked protein"
/note="VPg"
mat_peptide 2966..3508
/gene="POL"
/locus_tag="DC54_49757gpPOL"
/product="3C-like protease"
/note="3CLpro; calicivirin"
mat_peptide 3509..5038
/gene="POL"
/locus_tag="DC54_49757gpPOL"
/product="RNA-directed RNA polymerase"
gene 5022..6644
/gene="VP1"
/locus_tag="DC54_49757gpVP1"
CDS 5022..6644
/gene="VP1"
/locus_tag="DC54_49757gpVP1"
/codon_start=1
/product="capsid protein VP1"
/protein_id="AHX22035.1"
/translation="MKMASSDANPSDGSTANLVPEVNNEVMALEPVVGAAIAAPVAGQ
QNVIDPWIRNNFVQAPGGEFTVSPRNAPGEILWSAPLGPDLNPYLSHLARMYNGYAGG
FEVQVILAGNAFTAGKIIFAAVPPNFPTEGLSPSQVTMFPHIIVDVRQLEPVLIPLPD
VRNNFYHYNQSNDPTIKLIAMLYTPLRANNAGDDVFTVSCRVLTRPSPDFDFIFLVPP
TVESRTKPFSVPVLTVEEMTNSRFPIPLEKLFTGPSSAFVVQPQNGRCTTDGVLLGTT
QLSPVNICTFRGDVTHISGSRNYTMNLASQNWNSYDPTEEIPAPLGTPDFVGKIQGVL
TQTTRTDGSTRGHKATVYTGSADFSPKLGRVQFATDTDNDFVTNQNTKFTPVGVIQDG
STTPRNEPQQWVLPSYSGRNTHNVHLAPAVAPTFPGEQLLFFRSTMPGCSGYPNMDLD
CLLPQEWVQYFYQEAAPAQSDVALLRFVNPDTGRVLFECKLHKSGYVTVAHTGQHDLV
IPPNGYFRFDSWVNQFYTLAPMGNGTGRRRAL"
gene 6644..7450
/gene="VP2"
/locus_tag="DC54_49757gpVP2"
CDS 6644..7450
/gene="VP2"
/locus_tag="DC54_49757gpVP2"
/note="minor capsid protein"
/codon_start=1
/product="capsid protein VP2"
/protein_id="AHX22036.1"
/translation="MAGAFFAGLASDVLGSGLGSLINAGAGAINQKVEFENNRKLQQA
SFQFSSNLQQASFQHDKEMLQAQIEATKKLQQEMMRVKQAMLLEGGFSETDAARGAIN
APMTKTLDWSGTRYWAPDARTTTYNAGRFSTPQPSGALPGRASLRATVPARGSSSMSS
NSSIATSVYSNQTTSTRLGSTAGSGTSVSSLPSTARTRSWVEDQNRNLSPFMRGAHNI
SFVTPPSSISSSQGTVSTVPKEVLDSWTGAFNTRRQPLFAHIRKRGESRV"
ORIGIN
1 cgcaaaatct tcaagtgacg gagtgctttc tagcatggct gtcactttta aacgagccct
61 cggggcgcgg cctaaacagc ctcccccgag ggaaaaacca caaagacccc cacgaccacc
121 tactccagaa ctggttaaaa atattccccc tcccccaccc aacggagagg atgaaatagt
181 ggtttcttat agtgtcaaag atggtgtttc cggcttgcct gacctttcca ccgtcaggca
241 gccggaagaa tccaacacgg ccttcagtgt ccctccactc aatcagaggg agaatagaga
301 tgctaaggag ccactcactg gaacaattct ggaaatgtgg gacggggaaa tctaccatta
361 tggcctgtat gtggagcgag gtcttgtact aggtgtgcac aaaccgccag ctgccatcag
421 cctcgccaag gttgagctag caccactctc cttgtactgg agacctgtgt acactcctca
481 gtacctcatc tctccagaca ctctcaagaa actgtccgga gaaacgttcc cctacacagc
541 ctttgacaac aactgttatg ccttttgttg ctgggtcctg gacctaaatg actcgtggct
601 gagcaggaga atgatccaga gaacaactgg tttcttcagg ccctaccaag actggaatag
661 gaaacccctt cccactatgg atgactccaa aataaagaag gtagctaaca tatttctgtg
721 tgctctgtcc tcgctattca ccagacccat aaaagatata atagggaaga taaggcctct
781 taacatcctc aacatcttag cctcatgtga ttggaccttt gcgggcatag tggagtccct
841 gatactcttg gcagaactct ttggagtttt ctggacaccc ccagatgtgt ctgcgatgat
901 tgccccctta cttggtgact acgagctaca aggacctgag gaccttgcag tggagctcgt
961 ccccgtggtg atggggggaa ttggtttggt gctaggattc accaaagaga agattgggaa
1021 aatgttgtca tctgctgcgt ccactttgag agcttgcaaa gaccttggcg catatgggct
1081 agagatccta aagttagtca tgaagtggtt cttcccgaag aaggaggagg caaatgagct
1141 ggctatagtg aggtccatcg aggatgcagt cctggatctc gaggcaattg aaaacaatca
1201 tatgaccacc ttgcttaaag ataaagacag tctggcaacc tacatgagaa cacttgacct
1261 tgaagaggag aaagccagga aactctcaac caagtctgcc tcacccgaca tcgtgggcac
1321 aatcaacgcc ctcctggcga gaatcgctgc cgcacgttct ctggtgcatc gagcgaagga
1381 ggagctttcc agcagaccaa gacctgtggt gttgatgata tcaggcaggc caggaatagg
1441 gaagacccac ctcgctaggg aagtggctaa gagaatcgca gcctccctta caggagacca
1501 gcgtgtgggc ctcatcccac gcaatggcgt cgaccattgg gatgcgtaca agggggagag
1561 ggtcgtccta tgggacgatt atggaatgag caaccctatt cacgatgccc tcaggctgca
1621 agaactcgct gacacttgcc cccttactct gaactgtgac aggattgaaa ataaaggaaa
1681 ggtctttgac agcgatgtca tcattatcac cactaatctg gccaacccag caccactgga
1741 ctatgtcaac tttgaagcat gctcgaggcg cattgacttc ctcgtgtatg cagaagcccc
1801 tgaagtcgaa aaggcgaagc gtgacttccc aggccagcct gacatgtgga agaacgcttt
1861 cagttctgat ttctcacaca taaaactagc actggcccca cagggtggtt tcgacaagaa
1921 cgggaacacc ccacacggaa agggcgtcat gaagactctc accactggct cccttattgc
1981 ccgggcatca gggctactcc atgagaggtt agatgaattt gaactgcagg gcccagctct
2041 caccaccttc aatttcgatc gcaataaagt gcttgccttt agacagcttg ctgctgaaaa
2101 taaatatgga ttggtggaca caatgagggt tgggaaacag ctcaaggatg tcagaaccat
2161 gccagaactc aaacaagcac tcaagaatgt ctcaatcaag aagtgtcaaa tagtgtatag
2221 tggttgcacc tacatgcttg agtctgatgg caagggcaat gtgaaagttg acagaatcca
2281 aagcgccgcc gtgcagacca acaatgagct ggctggtgcc ttgcaccact tgaggtgcgc
2341 cagaatcaga tactatgtca agtgtgtcca ggaagccctg tattccatca tccaaatcgc
2401 tggggctgca tttgtcacca cgcgcattgc caagcgcatg aacatacaag acctatggtc
2461 caagccacaa gtggaaaaca cagaggagac taccagcaag gacgggtgcc caaaacctaa
2521 ggatgatgag gagtttgtca tttcatccga cgacatcaaa actgagggca agaaagggaa
2581 gaacaagact ggccgtggca agaagcacac agcattttca agtaaaggcc tcagtgatga
2641 agagtacgat gaatacaaga ggattagaga agaaaggaat ggcaagtact ctatagaaga
2701 gtaccttcag gacagggaca aatactatga ggaggtggcc attgccaggg cgactgagga
2761 agacttctgt gaagaggagg aggccaagat ccggcaaagg atctttaggc caacaaggaa
2821 acaacgcaag gaggaaagag tctctctcgg tctggtcaca ggctctgaaa ttaggaaaag
2881 aaacccagat gacttcaaac ccaaggggaa attgtgggct gacgatgaca ggagtgtgga
2941 ctacaatgag aaactcagtt ttgaggcccc accaagcatt tggtcgagaa tagtcaactt
3001 tggttcaggc tggggattct gggtttcccc cagtctgttc ataacatcaa cccatgttat
3061 accccagggc gcaaaggagt tctttggagt ccccatcaaa caaatacagg tacacaagtc
3121 aggcgagttc tgtcgcttga gattccctaa accaatcagg actgatgtga cgggcatgat
3181 cttagaagaa ggcgcacctg agggtaccgt ggtcacacta ctcatcaaaa ggtccactgg
3241 ggaactcatg cccctagcag ctaggatggg gacccatgcg accatgaaga tccaagggcg
3301 cactgttggg ggccagatgg gcatgcttct gacaggatcc aacgccaaga gcatggacct
3361 gggtactaca ccaggtgatt gtggctgccc ctatatctac aagagaggta atgactatgt
3421 ggtcattgga gtccacacgg ctgccgcacg tgggggaaac actgtcatat gtgccaccca
3481 ggggagtgaa ggagaggcta cacttgaggg tggtgacaac aagggaacat actgtggtgc
3541 accaatccta ggcccaggga gtgccccaac acttagcacc aagaccaaat tctggagatc
3601 gtccacagca tcactcccac ctggcaccta tgaaccagcc tatcttggtg gcaaggaccc
3661 tagagtcaag ggtggccctt cactgcagca agtcatgagg gaacagttga agccattcac
3721 agagcccagg ggcaagccac caaaaccaag tgtattagaa gctgccaaga agaccatcat
3781 taatgtcctt gagcaaacaa ttgatccacc tgagaaatgg tcgttcgcac aagcttgcgc
3841 gtcccttgac aagaccactt ccagtggtca tccgcaccac atgcggaaaa acgactgctg
3901 gaacggggag tccttcacag gcaagctggc agaccaggct tccaaggcca acctgatgtt
3961 tgaagaaggg aagaacatga ccccagtcta cacagctgcg ctcaaggatg agttagttaa
4021 aactgacaaa atttatggta agatcaagaa gaggcttctc tggggctcgg acttggcgac
4081 catgatccgg tgtgctcgag cattcggagg cctaatggat gaactcaaag cgcactgtgt
4141 cacacttccc attagagttg gcatgaatat gaatgaggat ggccccatca tcttcgagag
4201 gcattccagg tacacgtacc actatgatgc tgattactct cgatgggatt caacacaaca
4261 gagagccgtg ttggcagcag ctctagaaat catggttaaa ttctccccag aaccacattt
4321 ggctcaggta gtcgcggaag accttctttc tcctagcgtg gtggacgtgg gcgacttcac
4381 aatatcaatc aacgagggtc ttccctctgg ggtgccctgc acctcccaat ggaactccat
4441 cgcccactgg cttctcactc tctgtgcgct ctctgaagtc acaaacctat ctcctgatac
4501 catacaggct aattccctct tctcttttta tggtgatgat gaaattgtta gcacagacat
4561 aaaattggac ccagagaaat tgacagcaaa gctcagagaa tatgggttaa aaccaacccg
4621 ccctgacaaa actgaaggac cccttgtcat ctctgaagac ctgaatggcc taactttcct
4681 gcggagaact gtgacccgcg acccagctgg ttggtttgga aaactggagc agagttcaat
4741 actcaggcaa atgtactgga ctaggggtcc caaccatgga gacccatctg aaacaatgat
4801 tccacactcc caaagaccca tacaattgat gtccctactg ggggaggccg ctctccacgg
4861 cccagcattt tacagcaaaa tcagcaaatt ggtcattgca gagctaaaag aaggtggtat
4921 ggatttttac gtgcccagac aagagccaat gttcagatgg atgagattct cagatctgag
4981 cacgtgggag ggcgatcgca atctggctcc cagttttgtg aatgaagatg gcgtcgagtg
5041 acgccaaccc atctgatggg tccacagcca acctcgtccc agaggtcaac aatgaggtta
5101 tggctttgga gcccgtagtt ggtgctgcta ttgcggcacc tgtagcgggc cagcaaaatg
5161 taattgaccc ctggattaga aacaattttg tacaagcccc tggtggagag tttacagtat
5221 cccctagaaa cgctccaggt gagatactat ggagcgcgcc cttaggccct gatttgaatc
5281 cctacctttc ccatttggcc agaatgtaca atggttatgc aggtggtttt gaagtgcagg
5341 taatcctcgc ggggaatgcg ttcaccgccg ggaaaatcat atttgcagca gtcccaccaa
5401 atttcccaac tgaaggtttg agccccagcc aagtcactat gttcccccac ataatagtag
5461 atgttaggca attggaacct gtgttgatcc ccttacccga tgttaggaat aatttctacc
5521 attataatca gtcaaatgac cccaccatca aattgatagc aatgttgtac acaccactta
5581 gggctaacaa tgccggggac gacgtcttca cagtttcttg tcgagtcctc acgagaccat
5641 cccccgattt tgatttcata tttttggtgc cacccacagt tgaatcaaga actaaaccat
5701 tctctgtccc agttttaact gttgaggaga tgaccaattc aaggttcccc attcctttgg
5761 aaaagttgtt cacgggcccc agtagtgcct ttgttgttca accacaaaac ggcaggtgca
5821 cgactgatgg cgtgctccta ggtactaccc aactgtctcc tgtcaacatc tgcaccttca
5881 gaggggatgt cacccacatt tcaggcagtc gtaactacac aatgaatttg gcctcccaaa
5941 attggaacag ttacgatcca acagaagaaa tcccagcccc cctaggaact ccagatttcg
6001 tggggaagat tcaaggtgtg ctcacccaaa ccacaaggac agatggctcg acccgcggcc
6061 acaaagctac agtgtacact gggagcgccg acttttctcc aaaactgggt agggttcaat
6121 ttgccactga cacagacaat gattttgtaa ctaaccaaaa cacgaagttc accccagtcg
6181 gtgttatcca ggatggtagt actacccccc gaaacgaacc ccaacaatgg gtgctcccaa
6241 gttactcagg cagaaacact cataatgtgc acctggcccc cgctgtagcc cccactttcc
6301 cgggcgagca gctcctcttc ttcagatcta ccatgcccgg atgcagcggg taccccaaca
6361 tggatttgga ctgtctgctc ccccaggaat gggtgcagta tttctaccag gaggcagccc
6421 cagcacaatc tgatgtggcg ctgttaagat ttgtgaatcc agacacaggt agggttttgt
6481 ttgagtgtaa gcttcataaa tcaggctatg ttacagtggc tcacactggc caacatgatc
6541 tagttatccc ccccaatggt tattttagat ttgattcctg ggtcaaccag ttctacacac
6601 ttgcccccat gggaaatggg acggggcgta gacgtgcatt ataatggctg gggccttctt
6661 tgccggattg gcatctgacg tccttggctc tggacttggt tccctaatca atgctggggc
6721 tggggccatc aaccaaaaag ttgaatttga aaacaacaga aaattgcaac aagcctcctt
6781 ccaatttagt agcaatctac aacaggcttc ctttcaacat gacaaagaga tgctccaagc
6841 acaaattgag gccaccaaaa agttgcaaca ggaaatgatg agagttaaac aagcaatgct
6901 cctagagggt ggattctctg agacagatgc agcccgtggg gcaatcaacg cccccatgac
6961 aaaaactttg gactggagcg gaacaaggta ctgggctccc gatgctagga ctacaacata
7021 caatgcaggc cgcttttcca cccctcaacc ctcgggggca ctaccaggaa gagctagtct
7081 tagggctact gtccccgccc ggggttcctc cagcatgtct tctaactctt ctattgctac
7141 ttctgtgtat tcaaatcaaa ccacctcgac gagacttggt tctacagctg gttctggtac
7201 cagtgtctcg agcctcccgt caactgcaag gactaggagc tgggttgagg atcaaaatag
7261 gaatttgtca cctttcatga ggggggccca caacatctcg tttgtcaccc caccatctag
7321 catatcctct agccaaggca cagtctcaac cgtgcccaaa gaagttttgg actcctggac
7381 tggcgctttc aacacgcgca ggcagcctct cttcgctcac attcgcaagc gaggggagtc
7441 acgggtgtaa
//