Typing tool
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Typing tool
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Complete norovirus genomes
| KF712507 | GII.4 New Orleans | |
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| GII.P4 Apeldoorn |
ORF1: 1..5097
ORF2: 5078..6694
ORF3: 6694..7500
LOCUS KF712507 7500 bp ss-RNA linear VRL 26-OCT-2013
DEFINITION Norovirus Hu/GII.4/NIHIC1.11/2012/USA, partial genome.
ACCESSION KF712507
VERSION KF712507.1
DBLINK BioProject: PRJNA70471
KEYWORDS .
SOURCE Norovirus Hu/GII.4/NIHIC1.11/2012/USA
ORGANISM Norovirus Hu/GII.4/NIHIC1.11/2012/USA
Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
Picornavirales; Caliciviridae; Norovirus; Norovirus norwalkense.
REFERENCE 1 (bases 1 to 7500)
AUTHORS Madupu,R., Halpin,R.A., Ransier,A., Fedorova,N., Tsitrin,T.,
McLellan,M., Stockwell,T., Amedeo,P., Appalla,L., Bishop,B.,
Edworthy,P., Gupta,N., Hoover,J., Katzel,D., Li,K., Schobel,S.,
Shrivastava,S., Thovarai,V., Wang,S., Kim,M., Bok,K.,
Sosnovtsev,S.V., Wentworth,D.E. and Green,K.Y.
TITLE Direct Submission
JOURNAL Submitted (29-SEP-2013) J. Craig Venter Institute, 9704 Medical
Center Drive, Rockville, MD 20850, USA
COMMENT This work was supported by the National Institute of Allergy and
Infectious Diseases (NIAID), Genome Sequencing Centers for
Infectious Diseases (GSCID) program.
The genome sequence was generated using overlapping PCR amplicons
spanning the genome. The amplicons were pooled by sample and then
barcoded and sequenced using Next Generation Sequencing platforms.
The consensus sequences of the internal PCR primer hybridization
sites were manually verified using reads from amplicons that
spanned across the sites.
##Genome-Assembly-Data-START##
Current Finishing Status :: Finished
Assembly Method :: clc_ref_assemble_long v. 3.22.55705
Genome Coverage :: 197.2x
Sequencing Technology :: Sanger; Illumina
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7500
/organism="Norovirus Hu/GII.4/NIHIC1.11/2012/USA"
/mol_type="genomic RNA"
/strain="Hu/GII.4/NIHIC1.11/2012/USA"
/host="Homo sapiens"
/db_xref="taxon:1406806"
/geo_loc_name="USA: Maryland"
/collection_date="15-Oct-2012"
/PCR_primers="fwd_name: 1_Forward, fwd_seq:
gtgaatgawgatggcgt, rev_name: 1_Reverse, rev_seq:
ccagrctrtctttrtctt"
/PCR_primers="fwd_name: 2_Forward, fwd_seq:
ygactcgtggctgagyagga, rev_name: 2_Reverse, rev_seq:
gytgggccctgyagttcawa"
/PCR_primers="fwd_name: 3_Forward, fwd_seq:
tcaaccaartctgcttcacctg, rev_name: 3_Reverse, rev_seq:
aagtcytcctcrgtcgccct"
/PCR_primers="fwd_name: 4_Forward, fwd_seq:
gggaaraacaagrctggycg, rev_name: 4_Reverse, rev_seq:
crrctctytgttgtgttgaatccc"
/PCR_primers="fwd_name: 5_Forward, fwd_seq:
tggyaagatcaagaagaggc, rev_name: 5_Reverse, rev_seq:
atcagtcgtgcayctgccrt"
/PCR_primers="fwd_name: 6_Forward, fwd_seq:
cagtytcttgtcgrgtyctca, rev_name: 6_Reverse, rev_seq:
atcaatyttgtcttttcaca"
/note="genotype: GII.4"
gene <1..5097
/gene="POL"
/locus_tag="H649_46729gpPOL"
CDS <1..5097
/gene="POL"
/locus_tag="H649_46729gpPOL"
/note="genome polyprotein"
/codon_start=1
/product="putative nonstructural polyprotein"
/protein_id="AGX85933.1"
/translation="KMASNDASAAAVADSNNDTVKSSSDGVLSSMAVTFKRALGARPK
QPPPREIPQRPPRPPTPELIKNIPPPPPNGEDDVVVSYSVKDGISGLPDLSTVRQPEE
SNTAFSIPPLNQRESREAKEPLTGTILEMWDGEIYHYGLYVERGLVLGVHKPPAAISL
ARVELTPLSLYWRPVYTPQYLISPDALKRLHGETFPYTAFDNNCYAFCCWVLDLNDSW
LSRRMIQRTTGFFRPYQDWNRKPLPTMDDSKLKKVANIFLCALSSLFTRPIKDIIGKL
RPLNILNILASCDWSFAGIVESLILLAELFGVFWTPPDVSAMIAPLLGDYELQGPEDL
AVELVPVVMGGIGLVLGFTKEKIGKMLSSAASTLRACKDLGAYGLEILKLAVKWFFPK
KEEANELAIVRSIEDAVLDLEAIENNHMTTLLKDKSSLAAYMKTLDLEEEKARKLSTK
SASPDIVGTVNALLARIAAARSLVHRAKEELSSRPRPVVLMISGRPGIGKTYLAREVA
KRIAASLTGDQRVGLVPRNGVDHWDAYKGERVVLWDDYGMSNPIQDALRLQELADTCP
LTLNCDRIENKGKVFDSDVIIITTNLANPAPLDYVNFEACSRRIDFLAYAEAPEVEKA
KRDFPGQPDMWKNAFSPDFTHIKLTLAPQGGFDKNGNTPHGKGVMKTLTTGSLIARAS
GLLHERLDEFELQGPTLTTFNFDRNKVLAFRQLAAENKYGLMDTMKVGRQLKDVRTMP
ELKQALKNISIKKCQIVYSGSTYTLESDGKGNVKVDRVQSASVQTNNELAGALHHLRT
ARMRYYVRCVQEALYSIIQIAGAAFVTTRIVKRMNIQSLWSKPQVENTEETTSRDGCP
KPKDDEEFVISSDDIKTEGKKGKNKTGRGKKHTAFSSKGLSDEEYDEYKRIREERNGK
YSIEEYLQDRDKYYEEVAIARATEEDFCEEEEAKIRQRIFRPTRKQRKEERASLGLVT
GSEIRKRNPDDFKPKGKLWADDDRSVDYNEKLSFEAPPSIWSRIVNFGSGWGFWVSPS
LFITSTHVIPQGAKEFFGVPIKQIQVHKSGEFCRLRFPKPIRADVTGMILEEGAPEGT
VVTLLIKRSTGELMPLAARMGTHATMKIQGRTVGGQMGMLLTGSNAKSMDLGTTPGDC
GCPYIYKRGNDYVVIGVHTAAARGGNTVICATQGSEGEATLEGGDNKGTYCGAPILGP
GSAPKLSTKTKFWRSSTAPLPPGTYEPAYLGGRDPRVKGGPSLQQVMRDQLKPFTEPR
GKPPKPSVLEAAKKTIINVLEQTIDPPEKWSFAQACASLDKTTSSGHPHHMRKNDCWN
GESFTGKLADQASKANLMFEEGKNMTPVYTAALKDELVKTDKIYGKIKKRLLWGSDLA
TMIRCARAFGGLMDELKAHCVTLPIRVGMNMNEDGPIIFERHSRYTYHYDADYSRWDS
TQQRAVLAAALEIMVKFSPEPHLAQVVAEDLLSPSVVDVGDFTISINEGLPSGVPCTS
QWNSIAHWLLTLCALSEVTNLSPDTIQANSLFSFYGDDEIVSTDIKLDPEKLTAKLRE
YGLKPTRPDKTEGPLAISEDLDGLTFLRRTVTRDPAGWFGKLEQSSILRQMYWTRGSN
HEDPSETMIPHSQRPIQLMSLLGEAALHGPTFYSKISKLVIAELKEGGMDFYVPRQEP
MFRWMRFSDLSTWEGDRNLAPSFVNEDGVE"
mat_peptide <1..987
/gene="POL"
/locus_tag="H649_46729gpPOL"
/product="putative protein p48"
mat_peptide 988..2085
/gene="POL"
/locus_tag="H649_46729gpPOL"
/product="NTPase"
/note="p41"
mat_peptide 2086..2622
/gene="POL"
/locus_tag="H649_46729gpPOL"
/product="protein p22"
mat_peptide 2623..3021
/gene="POL"
/locus_tag="H649_46729gpPOL"
/product="viral genome-linked protein"
/note="VPg"
mat_peptide 3022..3564
/gene="POL"
/locus_tag="H649_46729gpPOL"
/product="3C-like protease"
/note="3CLpro; calicivirin"
mat_peptide 3565..5094
/gene="POL"
/locus_tag="H649_46729gpPOL"
/product="RNA-directed RNA polymerase"
gene 5078..6694
/gene="VP1"
/locus_tag="H649_46729gpVP1"
CDS 5078..6694
/gene="VP1"
/locus_tag="H649_46729gpVP1"
/codon_start=1
/product="capsid protein VP1"
/protein_id="AGX85934.1"
/translation="MKMASSDANPSDGSTANLVPEANNEVMALEPVVGAAIAAPVAGQ
QNVIDPWIRNNFVQAPGGEFTVSPRNAPGEVLWSAPLGPDLNPYLSHLARMYNGYAGG
FEVQVILAGNAFAAGKIIFAAVPPNFSTEGLSPSQVTMFPHLIVDVRQLEPVMIPLPD
IRNNFYHYNQSNDPTIKLIAMLYTPLRANNVGDDVFTVSCRVLTRPSPDFDFIFLVPP
TVESKTNPFSVPILTIEEMTNSRFPIPLEKLFTGPSSALVVQPQNGRCTTDGVLLGTT
QLSPVNICTFRGDVTHTTKTHTYQMNLAAQNWNSYDPTEEIPAPLGTPDFVGKIQGVL
TQTTKGNGSTRAHKATVYTGTVEFTPKLGRIQLFTDTDNDLEANQNTKFTPVGVIQDG
DHQNEPQQWVLPSYSGRNNHNVHLAPAVAPTFPGEQLLFFRSTMPGCSGYPNMNLDCL
LPQEWVQYFYQEAAPAQSDVALLRFVNPDTGRVLFECKLHKSGYVTVAHTGHHDLVIP
PNGYFRFDSWVSPFYTLAPMGNGTGRRRAL"
gene 6694..7500
/gene="VP2"
/locus_tag="H649_46729gpVP2"
CDS 6694..7500
/gene="VP2"
/locus_tag="H649_46729gpVP2"
/note="minor capsid protein"
/codon_start=1
/product="capsid protein VP2"
/protein_id="AGX85935.1"
/translation="MAGAFFAGLASDVLGSGLGSLINAGAGAINQKVEFENNRKLQQD
SFQFSSNLQQASFQHDKEMLQAQIEATKKLQQEMMRVKQAMLLEGGFSETDAARGAIN
APMTKVLDWSGTRYWAPGARTTTYNAGYFSTPQHSGALPGRANLRAVVPARGSSRVSS
NSSTATSVYSNQTASTRLGSTAGSGTSVSSLPSTARTRSWVEDQNRNLSPFMRGAHNI
SFVTPPSSRSSSQGTVSTVPKEVLNSQTGVFNTRRQPLFAHIRRRGESRA"
ORIGIN
1 aagatggcgt ctaacgacgc ttccgctgcc gctgttgctg acagcaacaa cgacaccgtt
61 aaatcttcaa gtgacggagt gctttctagc atggctgtca ctttcaaacg agccctcggg
121 gcgcggccta aacagcctcc cccgagggaa ataccacaaa gacccccgcg accacccact
181 ccagaactga ttaaaaatat cccccctccc ccacccaatg gagaggatga tgtagtggtt
241 tcttatagcg tcaaagatgg tatctctggt ttacctgatc tttccaccgt caggcaaccg
301 gaagaatcta atacggcctt cagtatccct ccactcaatc agagggagag tagagaagct
361 aaggaaccac tgactggaac aattttggaa atgtgggatg gagaaatcta tcattatggc
421 ctgtacgtag agcgaggtct tgtactaggt gtgcacaaac caccagctgc catcagtctc
481 gctagggtcg aactaacacc actttccttg tactggagac ctgtgtacac tccccagtac
541 ctcatctctc cagacgctct caagagacta cacggagaga cgttccccta cacagccttt
601 gacaataact gctatgcctt ttgttgttgg gtcctggacc taaacgactc gtggctgagt
661 aggagaatga tccagagaac aactggcttc ttcagaccct accaagattg gaataggaaa
721 cccctcccca ctatggatga ctccaaatta aagaaggtgg ctaacatatt cctgtgtgca
781 ttgtcttcgc tattcaccag gcctataaaa gatataatag ggaagctaag gcctcttaac
841 atccttaaca tcttggcttc atgtgattgg tcttttgcag gcatagtgga gtccttgata
901 ctcttggcag aactctttgg agttttctgg acgcccccag atgtgtctgc gatgattgcc
961 cccttactcg gtgactacga actgcaggga cctgaggacc ttgcagtgga gctcgtccct
1021 gtagtgatgg ggggaattgg tttggtgcta ggattcacca aagagaagat tggaaaaatg
1081 ttgtcatctg ctgcatccac cctgagagct tgtaaagatc ttggtgcata tgggctagag
1141 atcctaaagt tggctgtgaa gtggttcttc ccgaagaaag aggaagcaaa tgaactggct
1201 atagtgagat ccatcgagga tgcagtcctg gatctcgaag caattgaaaa caaccatatg
1261 accaccttgc tcaaagacaa aagcagtctg gcagcctaca tgaaaaccct tgaccttgag
1321 gaggagaaag ccaggaaact ctcaaccaag tctgcttcac ctgacattgt gggcacagtc
1381 aacgcccttc tggcgagaat cgctgctgca cgttctctgg tgcatcgagc gaaggaggaa
1441 ctttctagca gaccaagacc tgtagtgttg atgatatcag gcagaccagg aatagggaag
1501 acctaccttg ccagagaagt ggctaagaga atcgcagcct cccttacagg agaccagcgt
1561 gtaggcctcg tcccacgcaa tggcgtcgac cactgggatg cgtacaaggg ggagagggtc
1621 gttctatggg atgactatgg aatgagcaat cccatccaag atgccctcag attgcaagaa
1681 ctcgctgaca cttgtcccct caccctgaac tgtgacagga ttgagaacaa aggaaaggtc
1741 tttgacagcg atgtcatcat tatcaccact aacctggcta acccagcacc actggactat
1801 gtcaacttcg aagcatgctc gaggcgcatc gacttcctcg cgtatgcaga agcccctgag
1861 gtcgaaaagg cgaagcgtga cttcccaggc caacccgata tgtggaagaa cgccttcagt
1921 cctgatttca cacacataaa actaacattg gctccacagg gtggtttcga caagaacggg
1981 aacaccccac acgggaaggg cgtcatgaag actctcacca ccggctccct cattgcccgg
2041 gcatcagggc tactccatga gaggctagat gaatttgaac tacagggccc aactctcacc
2101 actttcaact ttgaccgcaa taaagtgctt gcctttagac aacttgctgc tgaaaacaaa
2161 tatgggttga tggacacaat gaaagttggg aggcagctca aggatgtcag aacaatgcca
2221 gagctcaagc aagcactcaa gaatatttca atcaagaagt gccagatagt gtacagtggt
2281 agcacctaca cacttgagtc tgatggcaag ggtaatgtga aggttgacag agtccaaagc
2341 gcctccgtgc agaccaacaa tgagctggct ggcgccctac accatctaag gacagccaga
2401 atgagatact atgtcaggtg tgtccaggag gccctgtatt ccatcatcca gattgctggg
2461 gctgcatttg tcaccacgcg catcgtcaag cgcatgaaca tacaaagcct atggtctaaa
2521 ccacaagtgg aaaacacaga ggagactacc agcagggacg ggtgtccaaa acccaaagat
2581 gatgaggagt tcgtcatttc atccgacgac atcaagactg aaggcaagaa agggaaaaac
2641 aagactggcc gcggtaagaa gcacacagcc ttctcaagca aaggtctcag tgatgaagag
2701 tacgatgagt acaagaggat tagagaagaa aggaatggta agtactccat agaagagtac
2761 cttcaagaca gggacaaata ctatgaggag gtggccattg ccagggcgac cgaggaagac
2821 ttctgtgaag aggaggaggc caagatccgg caaaggatct tcaggccaac aaggaaacaa
2881 cgcaaggagg aaagagcttc tctcggttta gtcacaggtt ctgaaattag gaaaagaaat
2941 ccagatgatt tcaaacccaa ggggaaattg tgggctgacg atgacagaag tgtggactac
3001 aatgagaaac tcagttttga agccccacca agcatctggt cgaggatagt caactttggt
3061 tcaggttggg gattctgggt ttcccccagt ttgttcataa catcaaccca tgtcataccc
3121 cagggagcaa aggagttctt tggagtcccc atcaaacaaa ttcaggtaca taagtcaggc
3181 gaattctgtc gcttgagatt cccaaaacca atcagggctg atgtgacggg catgatctta
3241 gaagaaggtg cacccgaggg caccgtggtt acactactca tcaaaaggtc cactggggaa
3301 ctcatgcccc tagcagctag gatggggacc catgcaacca tgaaaatcca aggacgcact
3361 gttggaggtc aaatgggcat gcttctgaca ggatccaacg ccaaaagcat ggacctgggt
3421 accacaccag gtgattgtgg ctgtccctac atctacaaga gaggaaatga ctatgtagtc
3481 attggagtcc acacggctgc cgctcgtggg ggcaacactg tcatctgtgc cacccagggg
3541 agtgagggag aggctacact tgaaggtggt gacaacaaag ggacatactg tggtgcacca
3601 atcctaggcc cagggagtgc cccaaaactt agcaccaaga ccaaattctg gagatcgtcc
3661 acagcaccac tcccacctgg cacctatgaa ccagcctatc ttggtggtag ggatcccaga
3721 gtcaagggtg ggccttcact acagcaggtc atgagggatc agctgaagcc attcacagag
3781 cccaggggca agccaccaaa gccaagtgtg ttggaagctg ctaagaaaac catcatcaat
3841 gtccttgagc aaacaattga tccacctgag aaatggtcgt tcgcacaagc ttgcgcgtca
3901 cttgataaga ccacctccag tggtcacccg caccacatgc ggaaaaacga ctgctggaac
3961 ggggagtcct tcacaggcaa gctggcagac caggcttcca aggccaacct gatgtttgaa
4021 gaggggaaga acatgacgcc agtctataca gctgcgctta aggatgaatt agttaaaact
4081 gacaagattt atggtaagat caagaaaagg cttctctggg gctcggattt ggcgaccatg
4141 atccggtgtg ctcgagcatt cggaggccta atggatgaac tcaaagcgca ctgtgtcaca
4201 cttcccatca gagttggtat gaatatgaat gaggatggcc ccatcatctt cgagaggcac
4261 tccaggtaca cgtaccacta tgatgctgat tactctcgat gggattcaac acaacagaga
4321 gccgtgttgg cagcagctct agaaattatg gttaagttct ccccagaacc acatttggcc
4381 caggtagtcg cagaagacct tctttcccct agcgtggtgg acgtgggtga ctttacaata
4441 tcaattaacg agggtcttcc ctctggagtg ccctgcacct cccaatggaa ctccatagcc
4501 cactggcttc tcaccctctg tgcgctctct gaagttacaa acttgtctcc tgacaccata
4561 caagctaatt ccctcttctc tttttatggt gatgatgaaa ttgttagcac agacataaaa
4621 ttggacccag aaaagttgac agcaaagctc agggaatatg ggttaaaacc aacccgcccc
4681 gacaaaactg aaggacccct tgccatctct gaagacttgg atggcctgac tttcttgcga
4741 agaactgtga cccgcgaccc agctggttgg tttggaaaac tggagcagag ctcaatactc
4801 aggcaaatgt actggactag gggttccaac catgaagacc catctgaaac aatgattcca
4861 cactcccaaa ggcccataca actgatgtcc ctactggggg aggccgcact ccacggccca
4921 acattctaca gcaaaatcag caagttagtc attgcagagc taaaagaagg tggcatggat
4981 ttttacgtgc ccagacaaga gccaatgttc agatggatga gattctcaga tctgagcacg
5041 tgggagggcg atcgcaatct ggctcccagt tttgtgaatg aagatggcgt cgagtgacgc
5101 caacccatct gatgggtcca cagccaacct cgtcccagag gccaacaatg aggttatggc
5161 tttggagccc gttgttggtg ccgccattgc ggcgcctgta gcgggccaac aaaatgtaat
5221 tgacccctgg attagaaaca attttgtgca agcccctggt ggagagttta cagtatcccc
5281 tagaaacgct ccaggtgaag tactatggag cgcgccctta ggccctgatt tgaatcccta
5341 cctttcccat ttggccagaa tgtacaatgg ttatgcaggt ggttttgaag tgcaggtaat
5401 cctcgcgggg aacgcgttcg ccgccggaaa aatcatattc gcagcagtcc caccaaattt
5461 ctcaactgaa ggcttgagcc ccagccaggt cactatgttc ccccacttaa tagtagatgt
5521 taggcaactg gaacctgtga tgatcccctt acccgatatt aggaataatt tctatcatta
5581 caatcaatca aatgatccca ccatcaaatt gatagcaatg ttgtatacac ctcttagggc
5641 taataatgtt ggtgacgatg tcttcacagt ttcttgtcgg gtactcacga gaccatcccc
5701 cgattttgat ttcatatttc tagtgccacc cacagttgaa tcaaaaacca acccattctc
5761 tgttccaatt ctaactattg aggagatgac caattcaaga ttccccattc ctttggaaaa
5821 gctgttcacg ggccccagca gtgcccttgt tgttcaacca caaaacggca ggtgcacgac
5881 tgatggcgtg ctcctgggta ccacccaact gtctcctgtc aacatctgca ccttcagagg
5941 ggatgtcacc catactacaa aaactcatac ctaccaaatg aatttggctg ctcaaaattg
6001 gaacagttat gacccaacag aagaaatccc agccccccta ggaactccag atttcgtggg
6061 aaagatccaa ggtgtgctca cccaaaccac gaagggaaat ggctcaaccc gtgcccacaa
6121 agctacagtg tacactggga ccgtcgagtt cactccaaaa ctgggtagaa tccaactttt
6181 cactgacaca gacaacgatc ttgaagctaa ccaaaacaca aagttcaccc cagtcggtgt
6241 catccaggat ggtgaccacc aaaatgaacc ccaacaatgg gtactcccaa gttactcagg
6301 tagaaataat cacaatgtgc acctggcccc cgctgtagcc cccacttttc cgggcgagca
6361 acttctcttc ttcagatcca ctatgcccgg atgcagcggg taccccaaca tgaatttgga
6421 ctgtctgctt ccccaggaat gggtgcaata tttctatcaa gaggcagccc cagcacaatc
6481 tgatgtggct ttgctaagat ttgtgaatcc agatacaggt agggttttgt ttgagtgcaa
6541 gcttcataaa tcaggttatg tcacagtggc tcacaccggc caccatgatt tggttatccc
6601 ccctaatggt tatttcagat ttgattcctg ggtcagcccc ttctacacac ttgcccccat
6661 gggaaatgga acggggcgta gacgtgcatt ataatggctg gagctttctt tgctggattg
6721 gcatctgacg tccttggatc cggacttggt tctctgatca atgccggggc tggggccatc
6781 aaccaaaaag ttgaatttga aaacaacaga aaattgcaac aagattcctt ccaatttagt
6841 agcaatctgc aacaggcttc ctttcaacat gataaagaga tgctccaagc acaaattgag
6901 gccaccaaaa agttgcaaca ggaaatgatg agagttaaac aggcaatgct cctagagggt
6961 gggttctctg agacagatgc agcccgtggg gcaatcaacg cccccatgac aaaggttttg
7021 gactggagtg ggacaaggta ctgggctccc ggtgctagga ctacaacata caatgcaggc
7081 tacttttcca ctccccaaca ctcgggggca ctgccaggaa gagctaatct tagggctgtt
7141 gtccccgctc ggggctcctc tagagtatct tctaattctt ctactgctac ctctgtgtat
7201 tcaaatcaaa ctgcttcaac gagacttggt tctacagctg gttctggtac cagtgtctcg
7261 agcctcccgt caactgcaag gactaggagt tgggttgagg atcaaaatag gaatttgtca
7321 cctttcatga ggggggccca caacatatcg tttgtcaccc caccatctag cagatcctct
7381 agtcaaggca cagtctcaac cgtgcccaaa gaagttttga actcccagac tggcgttttc
7441 aatacgcgca ggcagcctct ctttgctcac attcgtaggc gaggggagtc acgggcgtaa
//