Typing tool
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Typing tool
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Complete norovirus genomes
| KF712506 | GII.4 New Orleans | |
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| GII.P4 Hunter |
ORF1: 1..5099
ORF2: 5080..6696
ORF3: 6696..7502
LOCUS KF712506 7502 bp ss-RNA linear VRL 26-OCT-2013
DEFINITION Norovirus Hu/GII.4/NIHIC1.12/2012/USA, partial genome.
ACCESSION KF712506
VERSION KF712506.1
DBLINK BioProject: PRJNA70471
KEYWORDS .
SOURCE Norovirus Hu/GII.4/NIHIC1.12/2012/USA
ORGANISM Norovirus Hu/GII.4/NIHIC1.12/2012/USA
Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
Picornavirales; Caliciviridae; Norovirus; Norwalk virus.
REFERENCE 1 (bases 1 to 7502)
AUTHORS Madupu,R., Halpin,R.A., Ransier,A., Fedorova,N., Tsitrin,T.,
McLellan,M., Stockwell,T., Amedeo,P., Appalla,L., Bishop,B.,
Edworthy,P., Gupta,N., Hoover,J., Katzel,D., Li,K., Schobel,S.,
Shrivastava,S., Thovarai,V., Wang,S., Kim,M., Bok,K.,
Sosnovtsev,S.V., Wentworth,D.E. and Green,K.Y.
TITLE Direct Submission
JOURNAL Submitted (29-SEP-2013) J. Craig Venter Institute, 9704 Medical
Center Drive, Rockville, MD 20850, USA
COMMENT This work was supported by the National Institute of Allergy and
Infectious Diseases (NIAID), Genome Sequencing Centers for
Infectious Diseases (GSCID) program.
The genome sequence was generated using overlapping PCR amplicons
spanning the genome. The amplicons were pooled by sample and then
barcoded and sequenced using Next Generation Sequencing platforms.
The consensus sequences of the internal PCR primer hybridization
sites were manually verified using reads from amplicons that
spanned across the sites.
##Genome-Assembly-Data-START##
Current Finishing Status :: Finished
Assembly Method :: clc_ref_assemble_long v. 3.22.55705
Genome Coverage :: 195.8x
Sequencing Technology :: Sanger; Illumina
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7502
/organism="Norovirus Hu/GII.4/NIHIC1.12/2012/USA"
/mol_type="genomic RNA"
/strain="Hu/GII.4/NIHIC1.12/2012/USA"
/host="Homo sapiens"
/db_xref="taxon:1406807"
/country="USA: Maryland"
/collection_date="16-Oct-2012"
/PCR_primers="fwd_name: 1_Forward, fwd_seq:
gtgaatgawgatggcgt, rev_name: 1_Reverse, rev_seq:
ccagrctrtctttrtctt"
/PCR_primers="fwd_name: 2_Forward, fwd_seq:
ygactcgtggctgagyagga, rev_name: 2_Reverse, rev_seq:
gytgggccctgyagttcawa"
/PCR_primers="fwd_name: 3_Forward, fwd_seq:
tcaaccaartctgcttcacctg, rev_name: 3_Reverse, rev_seq:
aagtcytcctcrgtcgccct"
/PCR_primers="fwd_name: 4_Forward, fwd_seq:
gggaaraacaagrctggycg, rev_name: 4_Reverse, rev_seq:
crrctctytgttgtgttgaatccc"
/PCR_primers="fwd_name: 5_Forward, fwd_seq:
tggyaagatcaagaagaggc, rev_name: 5_Reverse, rev_seq:
atcagtcgtgcayctgccrt"
/PCR_primers="fwd_name: 6_Forward, fwd_seq:
cagtytcttgtcgrgtyctca, rev_name: 6_Reverse, rev_seq:
atcaatyttgtcttttcaca"
/note="genotype: GII.4"
gene <1..5099
/gene="POL"
/locus_tag="H649_46730gpPOL"
CDS <1..5099
/gene="POL"
/locus_tag="H649_46730gpPOL"
/note="genome polyprotein"
/codon_start=3
/product="putative nonstructural polyprotein"
/protein_id="AGX85930.1"
/translation="KMASNDASAAAVADSNNDTVKSSSDGVLSSMAVTFKRALGARPK
QPPPREIPQRPPRPPTPELIKNIPPPPPNGEDDVVVSYSVKDGISGLPDLSTVRQPEE
SNTAFSIPPLNQRESREAKEPLTGTILEMWDGEIYHYGLYVERGLVLGVHKPPAAISL
ARVELTPLSLYWRPVYTPQYLISPDALKRLHGETFPYTAFDNNCYAFCCWVLDLNDSW
LSRRMIQRTTGFFRPYQDWNRKPLPTMDDSKLKKVANIFLCALSSLFTRPIKDIIGKL
RPLNILNILASCDWSFAGIVESLILLAELFGVFWTPPDVSAMIAPLLGDYELQGPEDL
AVELVPVVMGGIGLVLGFTKEKIGKMLSSAASTLRACKDLGAYGLEILKLAVKWFFPK
KEEANELAIVRSIEDAVLDLEAIENNHMTTLLKDKSSLAAYMKTLDLEEEKARKLSTK
SASPDIVGTVNALLARIAAARSLVHRAKEELSSRPRPVVLMISGRPGIGKTYLAREVA
KRIAASLTGDQRVGLVPRNGVDHWDAYKGERVVLWDDYGMSNPIQDALRLQELADTCP
LTLNCDRIENKGKVFDSDVIIITTNLANPAPLDYVNFEACSRRIDFLAYAEAPEVEKA
KRDFPGQPDMWKNAFSPDFTHIKLTLAPQGGFDKNGNTPHGKGVMKTLTTGSLIARAS
GLLHERLDEFELQGPTLTTFNFDRNKVLAFRQLAAENKYGLMDTMKVGRQLKDVRTMP
ELKQALKNISIKKCQIVYSGSTYTLESDGKGNVKVDRVQSASVQTNNELAGALHHLRT
ARMRYYVRCVQEALYSIIQIAGAAFVTTRIVKRMNIQSLWSKPQVENTEETTSRDGCP
KPKDDEEFVISSDDIKTEGKKGKNKTGRGKKHTAFSSKGLSDEEYDEYKRIREERNGK
YSIEEYLQDRDKYYEEVAIARATEEDFCEEEEAKIRQRIFRPTRKQRKEERASLGLVT
GSEIRKRNPDDFKPKGKLWADDDRSVDYNEKLSFEAPPSIWSRIVNFGSGWGFWVSPS
LFITSTHVIPQGAKEFFGVPIKQIQVHKSGEFCRLRFPKPIRADVTGMILEEGAPEGT
VVTLLIKRSTGELMPLAARMGTHATMKIQGRTVGGQMGMLLTGSNAKSMDLGTTPGDC
GCPYIYKRGNDYVVIGVHTAAARGGNTVICATQGSEGEATLEGGDNKGTYCGAPILGP
GSAPKLSTKTKFWRSSTAPLPPGTYEPAYLGGRDPRVKGGPSLQQVMRDQLKPFTEPR
GKPPKPSVLEAAKKTIINVLEQTIDPPEKWSFAQACASLDKTTSSGHPHHMRKNDCWN
GESFTGKLADQASKANLMFEEGKNMTPVYTAALKDELVKTDKIYGKIKKRLLWGSDLA
TMIRCARAFGGLMDELKAHCVTLPIRVGMNMNEDGPIIFERHSRYTYHYDADYSRWDS
TQQRAVLAAALEIMVKFSPEPHLAQVVAEDLLSPSVVDVGDFTISINEGLPSGVPCTS
QWNSIAHWLLTLCALSEVTNLSPDTIQANSLFSFYGDDEIVSTDIKLDPEKLTAKLRE
YGLKPTRPDKTEGPLAISEDLDGLTFLRRTVTRDPAGWFGKLEQSSILRQMYWTRGSN
HEDPSETMIPHSQRPIQLMSLLGEAALHGPTFYSKISKLVIAELKEGGMDFYVPRQEP
MFRWMRFSDLSTWEGDRNLAPSFVNEDGVE"
mat_peptide <1..989
/gene="POL"
/locus_tag="H649_46730gpPOL"
/product="putative protein p48"
mat_peptide 990..2087
/gene="POL"
/locus_tag="H649_46730gpPOL"
/product="NTPase"
/note="p41"
mat_peptide 2088..2624
/gene="POL"
/locus_tag="H649_46730gpPOL"
/product="protein p22"
mat_peptide 2625..3023
/gene="POL"
/locus_tag="H649_46730gpPOL"
/product="viral genome-linked protein"
/note="VPg"
mat_peptide 3024..3566
/gene="POL"
/locus_tag="H649_46730gpPOL"
/product="3C-like protease"
/note="3CLpro; calicivirin"
mat_peptide 3567..5096
/gene="POL"
/locus_tag="H649_46730gpPOL"
/product="RNA-directed RNA polymerase"
primer_bind 1..13
gene 5080..6696
/gene="VP1"
/locus_tag="H649_46730gpVP1"
CDS 5080..6696
/gene="VP1"
/locus_tag="H649_46730gpVP1"
/codon_start=1
/product="capsid protein VP1"
/protein_id="AGX85931.1"
/translation="MKMASSDANPSDGSTANLVPEANNEVMALEPVVGAAIAAPVAGQ
QNVIDPWIRNNFVQAPGGEFTVSPRNAPGEVLWSAPLGPDLNPYLSHLARMYNGYAGG
FEVQVILAGNAFAAGKIIFAAVPPNFSTEGLSPSQVTMFPHLIVDVRQLEPVMIPLPD
IRNNFYHYNQSNDPTIKLIAMLYTPLRANNVGDDVFTVSCRVLTRPSPDFDFIFLVPP
TVESKTNPFSVPILTIEEMTNSRFPIPLEKLFTGPSSALVVQPQNGRCTTDGVLLGTT
QLSPVNICTFRGDVTHTTKTHTYQMNLAAQNWNSYDPTEEIPAPLGTPDFVGKIQGVL
TQTTKENGSTRAHKATVYTGTVEFTPKLGRIQLFTDTDNDLEANQNTKFTPVGVIQDG
DHQNEPQQWVLPSYSGRNNHNVHLAPAVAPTFPGEQLLFFRSTMPGCSGYPNMNLDCL
LPQEWVQYFYQEAAPAQSDVALLRFVNPDTGRVLFECKLHKSGYVTVAHTGHHDLVIP
PNGYFRFDSWVSPFYTLAPMGNGTGRRRAL"
gene 6696..7502
/gene="VP2"
/locus_tag="H649_46730gpVP2"
CDS 6696..7502
/gene="VP2"
/locus_tag="H649_46730gpVP2"
/note="minor capsid protein"
/codon_start=1
/product="capsid protein VP2"
/protein_id="AGX85932.1"
/translation="MAGAFFAGLASDVLGSGLGSLINAGAGAINQKVEFENNRKLQQD
SFQFSSNLQQASFQHDKEMLQAQIEATKKLQQEMMRVKQAMLLEGGFSETDAARGAIN
APMTKVLDWSGTRYWAPGARTTTYNAGYFSTPQHSGALPGRANLRAVVPARGSSRVSS
NSSTATSVYSNQTASTRLGSTAGSGTSVSSLPSTARTRSWVEDQNRNLSPFMRGAHNI
SFVTPPSSRSSSQGTVSTVPKEVLNSQTGVFNTRRQPLFAHIRRRGESRA"
ORIGIN
1 tgaagatggc gtctaacgac gcttccgctg ccgctgttgc tgacagcaac aacgacaccg
61 ttaaatcttc aagtgacgga gtgctttcta gcatggctgt cactttcaaa cgagccctcg
121 gggcgcggcc taaacagcct cccccgaggg aaataccaca aagacccccg cgaccaccca
181 ctccagaact gatcaaaaat atcccccctc ccccacccaa tggagaggat gatgtagtgg
241 tttcttatag cgtcaaagat ggtatctctg gtttacctga tctttccacc gtcaggcaac
301 cggaagaatc taatacggcc ttcagtatcc ctccactcaa tcagagggag agtagagaag
361 ctaaggaacc actgactgga acaattttgg aaatgtggga tggagaaatc tatcattatg
421 gcctgtacgt agagcgaggt cttgtactag gtgtgcacaa accaccagct gccatcagtc
481 tcgctagggt cgaactaaca ccactttcct tgtactggag acctgtgtac actccccagt
541 acctcatctc tccagacgct ctcaagagac tacacggaga gacgttcccc tacacagcct
601 ttgacaacaa ctgctatgcc ttttgttgtt gggtcctgga cctaaacgac tcgtggctga
661 gtaggagaat gatccagaga acaactggct tcttcagacc ctaccaagat tggaatagga
721 aacccctccc cactatggat gactccaaat taaagaaggt ggctaacata ttcctgtgtg
781 cattgtcttc gctattcacc aggcctataa aagatataat agggaagcta aggcctctta
841 acatccttaa catcttggct tcatgtgatt ggtcttttgc aggcatagtg gagtccttga
901 tactcttggc agaactcttt ggagttttct ggacgccccc agatgtgtct gcgatgattg
961 cccccttact cggtgactac gaactgcagg gacctgagga ccttgcagtg gagctcgtcc
1021 ctgtagtgat ggggggaatt ggtttggtgc taggattcac caaagagaag attggaaaaa
1081 tgttgtcatc tgctgcatcc accctgagag cttgtaaaga tcttggtgca tatgggctag
1141 agatcctaaa gttggctgtg aagtggttct tcccgaagaa agaggaagca aatgaactgg
1201 ctatagtgag atccatcgag gatgcagtcc tggatctcga agcaattgaa aacaaccata
1261 tgaccacctt gctcaaagac aaaagcagtc tggcagccta catgaaaacc cttgaccttg
1321 aggaggagaa agccaggaaa ctctcaacca agtctgcttc acctgacatt gtgggcacag
1381 tcaacgccct tctggcgaga atcgctgctg cacgttctct ggtgcatcga gcgaaggagg
1441 aactttctag cagaccaaga cctgtagtgt tgatgatatc aggcagacca ggaataggga
1501 agacctacct tgccagagaa gtggctaaga gaatcgcagc ctcccttaca ggagaccagc
1561 gtgtaggcct cgtcccacgc aatggcgtcg accactggga tgcgtacaag ggggagaggg
1621 tcgttctatg ggatgactat ggaatgagca atcccatcca agatgccctc agattgcaag
1681 aactcgctga cacttgtccc ctcaccctga actgtgacag gattgagaac aaaggaaagg
1741 tctttgacag cgatgtcatc attatcacca ctaacctggc taacccagca ccactggact
1801 atgtcaactt cgaagcatgc tcgaggcgca tcgacttcct cgcgtatgca gaagcccctg
1861 aggtcgaaaa ggcgaagcgt gacttcccag gccaacccga tatgtggaag aacgccttca
1921 gtcctgattt cacacacata aaactaacat tggctccaca gggtggtttc gacaagaacg
1981 ggaacacccc acacgggaag ggcgtcatga agactctcac caccggctcc ctcattgccc
2041 gggcatcagg gctactccat gagaggctag atgaatttga actacagggc ccaactctca
2101 ccactttcaa ctttgaccgc aataaagtgc ttgcctttag acaacttgct gctgaaaaca
2161 aatatgggtt gatggacaca atgaaagttg ggaggcagct caaggatgtc agaacaatgc
2221 cagagctcaa gcaagcactc aagaatattt caatcaagaa gtgccagata gtgtacagtg
2281 gtagcaccta cacacttgag tctgatggca agggtaatgt gaaggttgac agagtccaaa
2341 gcgcctccgt gcagaccaac aatgagctgg ctggcgccct acaccatcta aggacagcca
2401 gaatgagata ctatgtcagg tgtgtccagg aggccctgta ttccatcatc cagattgctg
2461 gggctgcatt tgtcaccacg cgcatcgtca agcgcatgaa catacaaagc ctatggtcta
2521 aaccacaagt ggaaaacaca gaggagacta ccagcaggga cgggtgtcca aaacccaaag
2581 atgatgagga gttcgtcatt tcatccgacg acatcaagac tgaaggcaag aaagggaaaa
2641 acaagactgg ccgcggtaag aagcacacag ccttctcaag caaaggtctc agtgatgaag
2701 agtacgatga gtacaagagg attagagaag aaaggaatgg taagtactcc atagaagagt
2761 accttcaaga cagggacaaa tactatgagg aggtggccat tgccagggcg accgaggaag
2821 acttctgtga agaggaggag gccaagatcc ggcaaaggat cttcaggcca acaaggaaac
2881 aacgcaagga ggaaagagct tctctcggtt tagtcacagg ttctgaaatt aggaaaagaa
2941 atccagatga tttcaaaccc aaggggaaat tgtgggctga cgatgacaga agtgtggact
3001 acaatgagaa actcagtttt gaagccccac caagcatctg gtcgaggata gtcaactttg
3061 gttcaggttg gggattctgg gtttccccca gtttgttcat aacatcaacc catgtcatac
3121 cccagggagc aaaggagttc tttggagtcc ccatcaaaca aattcaggta cataagtcag
3181 gcgaattctg tcgcttgaga ttcccaaaac caatcagggc tgatgtgacg ggcatgatct
3241 tagaagaagg tgcacccgag ggcaccgtgg ttacactact catcaaaagg tccactgggg
3301 aactcatgcc cctagcagct aggatgggga cccatgcaac catgaaaatc caaggacgca
3361 ctgttggagg tcaaatgggc atgcttctga caggatccaa cgccaaaagc atggacctgg
3421 gtaccacacc aggtgattgt ggctgtccct acatctacaa gagaggaaat gactatgtag
3481 tcattggagt ccacacggct gccgctcgtg ggggcaacac tgtcatctgt gccacccagg
3541 ggagtgaggg agaggctaca cttgaaggtg gtgacaacaa agggacatac tgtggtgcac
3601 caatcctagg cccagggagt gccccaaaac ttagcaccaa gaccaaattc tggagatcgt
3661 ccacagcacc actcccacct ggcacctatg aaccagccta tcttggtggt agggatccca
3721 gagtcaaggg tgggccttca ctacagcagg tcatgaggga tcagctgaag ccattcacag
3781 agcccagggg caagccacca aagccaagtg tgttggaagc tgctaagaaa accatcatca
3841 atgtccttga gcaaacaatt gatccacctg agaaatggtc gttcgcacaa gcttgcgcgt
3901 cacttgataa gaccacctcc agtggtcacc cgcaccacat gcggaaaaac gactgctgga
3961 acggggagtc cttcacaggc aagctggcag accaggcttc caaggccaac ctgatgtttg
4021 aagaggggaa gaacatgacg ccagtctata cagctgcgct taaggatgaa ttagttaaaa
4081 ctgacaagat ttatggtaag atcaagaaaa ggcttctctg gggctcggat ttggcgacca
4141 tgatccggtg tgctcgagca ttcggaggcc taatggatga actcaaagcg cactgtgtca
4201 cacttcccat cagagttggt atgaatatga atgaggatgg ccccatcatc ttcgagaggc
4261 actccaggta cacgtaccac tatgatgctg attactctcg atgggattca acacaacaga
4321 gagccgtgtt ggcagcagct ctagaaatta tggttaagtt ctccccagaa ccacatttgg
4381 cccaggtagt cgcagaagac cttctttccc ctagcgtggt ggacgtgggt gactttacaa
4441 tatcaattaa cgagggtctt ccctctggag tgccctgcac ctcccaatgg aactccatag
4501 cccactggct tctcaccctc tgtgcgctct ctgaagttac aaacttgtct cctgacacca
4561 tacaagctaa ttccctcttc tctttttatg gtgatgatga aattgttagc acagacataa
4621 aattggaccc agaaaagttg acagcaaagc tcagggaata tgggttaaaa ccaacccgcc
4681 ccgacaaaac tgaaggaccc cttgccatct ctgaagactt ggatggcctg actttcttgc
4741 gaagaactgt gacccgcgac ccagctggtt ggtttggaaa actggagcag agctcaatac
4801 tcaggcaaat gtactggact aggggttcca accatgaaga cccatctgaa acaatgattc
4861 cacactccca aaggcccata caactgatgt ccctactggg ggaggccgca ctccacggcc
4921 caacattcta cagcaaaatc agcaagttag tcattgcaga gctaaaagaa ggtggcatgg
4981 atttttacgt acccagacaa gagccaatgt tcagatggat gagattctca gatctgagca
5041 cgtgggaggg cgatcgcaat ctggctccca gttttgtgaa tgaagatggc gtcgagtgac
5101 gccaacccat ctgatgggtc cacagccaac ctcgtcccag aggccaacaa tgaggttatg
5161 gctttggagc ccgttgttgg tgccgccatt gcggcgcctg tagcgggcca acaaaatgta
5221 attgacccct ggattagaaa caattttgtg caagcccctg gtggagagtt tacagtatcc
5281 cctagaaacg ctccaggtga agtactatgg agcgcgccct taggccctga tttgaatccc
5341 tacctttccc atttggccag aatgtacaat ggttatgcag gtggttttga agtgcaggta
5401 atcctcgcgg ggaacgcgtt cgccgccgga aaaatcatat tcgcagcagt cccaccaaat
5461 ttctcaactg aaggcttgag ccccagccag gtcactatgt tcccccactt aatagtagat
5521 gttaggcaac tggaacctgt gatgatcccc ttacccgata ttaggaataa tttctatcat
5581 tacaatcaat caaatgatcc caccatcaaa ttgatagcaa tgttgtatac acctcttagg
5641 gctaataatg ttggtgacga tgtcttcaca gtttcttgtc gggtactcac gagaccatcc
5701 cccgattttg atttcatatt tctagtgcca cccacagttg aatcaaaaac caacccattc
5761 tctgttccaa ttctaactat tgaggagatg accaattcaa gattccccat tcctttggaa
5821 aagctgttca cgggccccag cagtgccctt gttgttcaac cacaaaacgg caggtgcacg
5881 actgatggcg tgctcctggg taccacccaa ctgtctcctg tcaacatctg caccttcaga
5941 ggggatgtca cccatactac aaaaactcat acctaccaaa tgaatttggc tgctcaaaat
6001 tggaacagtt atgacccaac agaagaaatc ccagcccccc taggaactcc agatttcgtg
6061 ggaaagatcc aaggtgtgct cacccaaacc acgaaggaaa atggctcaac ccgtgcccac
6121 aaagctacag tgtacactgg gaccgtcgag ttcactccaa aactgggtag aatccaactt
6181 ttcactgaca cagacaacga tcttgaagct aaccaaaaca caaagttcac cccagtcggt
6241 gtcatccagg atggtgacca ccaaaatgaa ccccaacaat gggtactccc aagttactca
6301 ggtagaaata atcacaatgt gcacctggcc cccgctgtag cccccacttt tccgggcgag
6361 caacttctct tcttcagatc cactatgccc ggatgcagcg ggtaccccaa catgaatttg
6421 gactgtctgc ttccccagga atgggtgcaa tatttctatc aagaggcagc cccagcacaa
6481 tctgatgtgg ctttgctaag atttgtgaat ccagatacag gtagggtttt gtttgagtgc
6541 aagcttcata aatcaggtta tgtcacagtg gctcacaccg gccaccatga tttggttatc
6601 ccccctaatg gttatttcag atttgattcc tgggtcagcc ccttctacac acttgccccc
6661 atgggaaatg gaacggggcg tagacgtgca ttataatggc tggagctttc tttgctggat
6721 tggcatctga cgtccttgga tccggacttg gttctctgat caatgccggg gctggggcca
6781 tcaaccaaaa agttgaattt gaaaacaaca gaaaattgca acaagattcc ttccaattta
6841 gtagcaatct gcaacaggct tcctttcaac atgataaaga gatgctccaa gcacaaattg
6901 aggccaccaa aaagttgcaa caggaaatga tgagagttaa acaggcaatg ctcctagagg
6961 gtgggttctc tgagacagat gcagcccgtg gggcaatcaa cgcccccatg acaaaggttt
7021 tggactggag tgggacaagg tactgggctc ccggtgctag gactacaaca tacaatgcag
7081 gctacttttc cactccccaa cactcggggg cactgccagg aagagctaat cttagggctg
7141 ttgtccccgc tcggggctcc tctagagtat cttctaattc ttctactgct acctctgtgt
7201 attcaaatca aactgcttca acgagacttg gttctacagc tggttctggt accagtgtct
7261 cgagcctccc gtcaactgca aggactagga gttgggttga ggatcaaaat aggaatttgt
7321 cacctttcat gaggggggcc cacaacatat cgtttgtcac cccaccatct agcagatcct
7381 ctagtcaagg cacagtctca accgtgccca aagaagtttt gaactcccag actggcgttt
7441 tcaatacgcg caggcagcct ctctttgctc acattcgtag gcgaggggag tcacgggcgt
7501 aa
//