Typing tool
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Typing tool
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Complete norovirus genomes
| KF712497 | GII.4 Den Haag | |
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| GII.P4 New Orleans |
ORF1: 1..5100
ORF2: 5081..6703
ORF3: 6703..7509
LOCUS KF712497 7509 bp ss-RNA linear VRL 26-OCT-2013
DEFINITION Norovirus Hu/GII.4/NIHIC27.2/2012/USA, complete genome.
ACCESSION KF712497
VERSION KF712497.1
DBLINK BioProject: PRJNA70471
KEYWORDS .
SOURCE Norovirus Hu/GII.4/NIHIC27.2/2012/USA
ORGANISM Norovirus Hu/GII.4/NIHIC27.2/2012/USA
Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
Picornavirales; Caliciviridae; Norovirus; Norwalk virus.
REFERENCE 1 (bases 1 to 7509)
AUTHORS Madupu,R., Halpin,R.A., Ransier,A., Fedorova,N., Tsitrin,T.,
McLellan,M., Stockwell,T., Amedeo,P., Appalla,L., Bishop,B.,
Edworthy,P., Gupta,N., Hoover,J., Katzel,D., Li,K., Schobel,S.,
Shrivastava,S., Thovarai,V., Wang,S., Kim,M., Bok,K.,
Sosnovtsev,S.V., Wentworth,D.E. and Green,K.Y.
TITLE Direct Submission
JOURNAL Submitted (29-SEP-2013) J. Craig Venter Institute, 9704 Medical
Center Drive, Rockville, MD 20850, USA
COMMENT This work was supported by the National Institute of Allergy and
Infectious Diseases (NIAID), Genome Sequencing Centers for
Infectious Diseases (GSCID) program.
The genome sequence was generated using overlapping PCR amplicons
spanning the genome. The amplicons were pooled by sample and then
barcoded and sequenced using Next Generation Sequencing platforms.
The consensus sequences of the internal PCR primer hybridization
sites were manually verified using reads from amplicons that
spanned across the sites.
Genome sequence lacks part of non-coding region.
##Genome-Assembly-Data-START##
Current Finishing Status :: Finished
Assembly Method :: clc_ref_assemble_long v. 3.22.55705
Genome Coverage :: 200.3x
Sequencing Technology :: Sanger; Illumina
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7509
/organism="Norovirus Hu/GII.4/NIHIC27.2/2012/USA"
/mol_type="genomic RNA"
/strain="Hu/GII.4/NIHIC27.2/2012/USA"
/host="Homo sapiens"
/db_xref="taxon:1406800"
/country="USA: Maryland"
/collection_date="06-Apr-2012"
/PCR_primers="fwd_name: 1_Forward, fwd_seq:
gtgaatgawgatggcgt, rev_name: 1_Reverse, rev_seq:
ttggttgagagyttyctg"
/PCR_primers="fwd_name: 2_Forward, fwd_seq:
ccgcaaaatcttcaagtg, rev_name: 2_Reverse, rev_seq:
cwacaggtcttggtctgctrga"
/PCR_primers="fwd_name: 3_Forward, fwd_seq:
tcaaccaartctgcttcacctg, rev_name: 3_Reverse, rev_seq:
ratcctttgccggatcttgg"
/PCR_primers="fwd_name: 4_Forward, fwd_seq:
ggcaagaagcacacagcctt, rev_name: 4_Reverse, rev_seq:
crrctctytgttgtgttgaatccc"
/PCR_primers="fwd_name: 5_Forward, fwd_seq:
tggyaagatcaagaagaggc, rev_name: 5_Reverse, rev_seq:
acaacaaargcacygctrgg"
/PCR_primers="fwd_name: 6_Forward, fwd_seq:
gagrccrtccccygattttg, rev_name: 6_Reverse, rev_seq:
atcaatyttgtcttttcaca"
/note="genotype: GII.4"
gene 1..5100
/gene="POL"
/locus_tag="H649_46749gpPOL"
CDS 1..5100
/gene="POL"
/locus_tag="H649_46749gpPOL"
/note="genome polyprotein"
/codon_start=1
/product="nonstructural polyprotein"
/protein_id="AGX85903.1"
/translation="MKMASNDASAAAVANSNNDTAKSSSDGVLSSMAVTFKRALGARP
KQPPPREKPQRPPRPPTPELVKNIPPPPPNGEDEIVVSYSVKDGVSGLPDLSTVRQPE
ESNTAFSVPPLNQRENRDAKEPLTGTILEMWDGEIYHYGLYVERGLVLGVHKPPAAIS
LAKVELAPLSLYWRPVYTPQYLISPDTLKKLSGETFPYTAFDNNCYAFCCWVLDLNDS
WLSRRMIQRTTGFFRPYQDWNRKPLPTMDDSKIKKVANIFLCALSSLFTRPIKDIIGK
IRPLNILNILASCDWTFAGIVESLILLAELFGVFWTPPDVSAMIAPLLGDYELQGPED
LAVELVPVVMGGIGLVLGFTKEKIGKMLSSAASTLRACKDLGAYGLEILKLVMKWFFP
KKEEANELAIVRSIEDAVLDLEAIENNHMTTLLKDKDSLATYMRTLDLEEEKARKLST
KSASPDIVGTINALLARIAAARSLVHRAKEELSSRPRPVVLMISGRPGIGKTHLAREV
AKRIAASLTGDQRVGLIPRNGVDHWDAYKGERVVLWDDYGMSNPIHDALRLQELADTC
PLTLNCDRIENKGKVFDSDVIIITTNLANPAPLDYVNFEACSRRIDFLVYAEAPDVEK
AKRDFPGQPDMWKNAFSSDFSHIKLALAPQGGFDKNGNTPHGRGVMKTLTTGSLIARA
SGLLHERLDEFELQGPALTTFNFDRNKVLAFRQLAAENKYGLMDTMRVGKQLKDVRTM
PELKQALKNVSIKKCQIVYSGCTYMLESDGKGNVKVDRIQSAAVQTNNELAGALHHLR
CARIRYYVKCVQEALYSIIQIAGAAFVTTRIAKRMNIQDLWSKPQVENTEETTSKDGC
PKPKDDEEFVISSDDIKTEGKKGKNKTGRGKKHTAFSSKGLSDEEYDEYKRIREERNG
KYSIEEYLQDRDKYYEEVAIARATEEDFCEEEEAKIRQRIFRPTRKQRKEERASLGLV
TGSEIRKRNPDDFKPKGKLWADDDRSVDYNEKLSFEAPPSIWSRIVNFGSGWGFWVSP
SLFITSTHVIPQGAKEFFGVPIKQIQVHKSGEFCRLRFPKPIRTDVTGMILEEGAPEG
TVVTLLIKRPTGELMPLAARMGTHATMKIQGRTVGGQMGMLLTGSNAKSMDLGTTPGD
CGCPYIYKRGNDYVVIGVHTAAARGGNTVICATQGSEGEATLEGGDNKGTYCGAPILG
PGSAPTLSTKTKFWRSSTASLPPGTYEPAYLGGKDPRVKGGPSLQQVMREQLKPFTEP
RGKPPKPSVLEAAKKTIINVLEQTIDPPEKWSFAQACASLDKTTSSGHPHHMRKNDCW
NGESFTGKLADQASKANLMFEEGKNMTPVYTAALKDELVKTDKIYGKIKKRLLWGSDL
ATMIRCARAFGGLMDELKAHCVTLPIRVGMNMNEDGPIIFERHSRYTYHYDADYSRWD
STQQRAVLAAALEIMVKFSPEPQLAQVVAEDLLSPSVVDVGDFTISINEGLPSGVPCT
SQWNSIAHWLLTLCALSEVTNLSPDTIQANSLFSFYGDDEIVSTDIKLDPEKLTTKLR
EYGLKPTRPDKTEGPLVISEDLNGLTFLRRTVTRDPAGWFGKLEQSSILRQMYWTRGP
NHGDPSETMIPHSQRPIQLMSLLGEAALHGPAFYSKISKLVIAELKEGGMDFYVPRQE
PMFRWMRFSDLSTWEGDRNLAPSFVNEDGVE"
mat_peptide 1..990
/gene="POL"
/locus_tag="H649_46749gpPOL"
/product="protein p48"
mat_peptide 991..2088
/gene="POL"
/locus_tag="H649_46749gpPOL"
/product="NTPase"
/note="p41"
mat_peptide 2089..2625
/gene="POL"
/locus_tag="H649_46749gpPOL"
/product="protein p22"
mat_peptide 2626..3024
/gene="POL"
/locus_tag="H649_46749gpPOL"
/product="viral genome-linked protein"
/note="VPg"
mat_peptide 3025..3567
/gene="POL"
/locus_tag="H649_46749gpPOL"
/product="3C-like protease"
/note="3CLpro; calicivirin"
mat_peptide 3568..5097
/gene="POL"
/locus_tag="H649_46749gpPOL"
/product="RNA-directed RNA polymerase"
primer_bind 1..13
gene 5081..6703
/gene="VP1"
/locus_tag="H649_46749gpVP1"
CDS 5081..6703
/gene="VP1"
/locus_tag="H649_46749gpVP1"
/codon_start=1
/product="capsid protein VP1"
/protein_id="AGX85904.1"
/translation="MKMASSDANPSDGSAANLVPEVNNEVMALEPVVGAAIAAPVAGQ
QNVIDPWIRSNFVQAPGGEFTVSPRNAPGEILWSAPLGPDLNPYLSHLARMYNGYAGG
FEVQVILAGNAFTAGKIIFAAVPPNFPTEGLSPSQVTMFPHIIVDVRQLEPVLIPLPD
VRNNFYHYNQSNDSTIKLIAMLYTPLRANNAGDDVFTVSCRVLTRPSPDFDFIFLVPP
TVESRTKPFTVPILTVEEMTNSRFPIPLEKLFTGPSSAFVVQPQNGRCTTDGVLLGTT
QLSPVNICTFRGDVTHIAGSRNYTMNLASLNWSNYDPTEEIPAPLGTPDFVGKIQGVL
TQTTKGDGSTRGHKATVYTGSADFTPKLGSIQFATDTENDFETHQNTRFTPVGVIQDG
GTTHRNEPQQWVLPSYSGRNVHNVHLAPAVAPNFPGEQLLFFRSTMPGCSGYPNMDLD
CLLPQEWVQHFYQEAAPAQSDVALLRFVNPDTGRVLFECKLHKSGYVTVAHTGQHDLV
IPPNGYFRFDSWVNQFYTLAPMGNGTGRRRAL"
gene 6703..7509
/gene="VP2"
/locus_tag="H649_46749gpVP2"
CDS 6703..7509
/gene="VP2"
/locus_tag="H649_46749gpVP2"
/note="minor capsid protein"
/codon_start=1
/product="capsid protein VP2"
/protein_id="AGX85905.1"
/translation="MAGAFFAGLASDVLSSGLGSLINAGAGAINQRIDFENNRKLQQA
SFQFSSNLQQASFQHDKEMLQAQIEATKKLQQELMKVKQAMLLEGGFSETDAARGAIN
APMTKALDWSGTRYWAPDARTTTYNAGRFSTPQPSGALPGRINPRTPIPARGSPSISS
NVSTANSIHSNQTASTRLGSTAGSGTDVSSLPSTARTRSWVEDQNRNLSPFMRGAHNI
SFVTPPSSRSSSQGTVSTVPKDVLDSWTGAFNTRRQPLFAHIRRRGESRV"
ORIGIN
1 atgaagatgg cgtctaacga cgcttccgct gccgctgttg ctaacagcaa caacgacacc
61 gcaaaatctt caagtgacgg agtgctttct agcatggctg tcacttttaa acgagccctc
121 ggggcgcggc ctaaacagcc tcccccgagg gaaaaacccc aaagaccccc acgaccacct
181 actccagaac tggttaaaaa tattccccct cccccaccca acggagagga tgaaatagtg
241 gtttcttata gtgtcaaaga tggtgtttcc ggcttgcctg acctttccac cgtcaggcaa
301 ccggaagaat ccaacacggc cttcagtgtc cctccactca atcagaggga gaatagagat
361 gctaaggagc cactcactgg aacaattctg gaaatgtggg acggggaaat ctaccattac
421 ggcctgtatg tggagcgagg tcttgtacta ggtgtgcaca aaccgccagc tgccatcagc
481 ctcgctaagg ttgagctagc accactctcc ttgtactgga gacctgtgta cactcctcag
541 tacctcatct ctccagacac actcaagaaa ttgtccggag aaacgttccc ctacacagcc
601 tttgacaaca actgttatgc cttttgttgc tgggtcctgg acctaaatga ctcgtggctg
661 agcaggagaa tgatccagag gacaactggt ttcttcaggc cctaccaaga ctggaatagg
721 aaaccccttc ccactatgga tgactccaaa ataaagaagg tagctaacat atttctgtgt
781 gctctgtcct cgctattcac cagacccata aaagatataa tagggaagat aaggcctctt
841 aacatcctca acatcttagc ctcatgtgac tggacctttg cgggcatagt ggagtccctg
901 atactcttgg cagaactctt tggagttttc tggacacccc cagatgtgtc tgcgatgatt
961 gcccccttac ttggtgacta cgagctacaa ggacctgagg accttgcggt ggagctcgtc
1021 cccgtggtga tggggggaat tggtttggtg ctaggattca ccaaagagaa gattgggaaa
1081 atgttgtcat ctgctgcgtc caccttgaga gcttgcaaag accttggtgc atatgggcta
1141 gagatcctaa agttagtcat gaagtggttc ttcccgaaga aggaggaggc aaatgagctg
1201 gctatagtga ggtccatcga ggatgcagtc ctggatctcg aggcaattga aaacaatcat
1261 atgaccacct tactcaaaga taaagacagt ctggcaacct acatgagaac acttgacctt
1321 gaagaggaga aagccaggaa actctcaacc aagtctgcct cacccgacat cgtgggcaca
1381 atcaacgctc tcctggcgag aatcgccgcc gcacgttctc tggtgcatcg agcgaaggag
1441 gagctttcca gcagaccaag acctgtggtg ttgatgatat caggcaggcc aggaataggg
1501 aagacccacc tcgctaggga agtggctaag agaatcgcag cctcccttac aggagaccag
1561 cgtgtgggcc tcatcccacg caatggcgtc gaccattggg atgcgtacaa gggggagagg
1621 gtcgtcctat gggacgatta tggaatgagc aaccctattc acgacgccct caggctgcaa
1681 gaactcgctg acacttgccc cctcactcta aactgtgaca ggattgaaaa taaaggaaag
1741 gtctttgaca gcgatgtcat cattatcact actaatctgg ccaacccagc accactggac
1801 tatgtcaact ttgaagcatg ctcgaggcgc attgacttcc tcgtgtatgc agaagcccct
1861 gatgtcgaaa aggcgaagcg tgacttccca ggccaacctg acatgtggaa gaatgctttc
1921 agttctgatt tctcacacat aaaactagca ctggccccac agggtggttt cgacaagaac
1981 gggaacaccc cacacggaag gggcgtcatg aagactctca ccactggttc ccttattgcc
2041 cgggcatcag ggctactcca tgagaggtta gatgaatttg aactgcaggg cccagctctc
2101 accaccttca acttcgatcg caataaagtg cttgccttta gacagcttgc tgctgaaaat
2161 aaatatggat tgatggacac aatgagggtt gggaaacagc tcaaggatgt cagaaccatg
2221 ccagaactca aacaagcact caagaatgtc tcaatcaaga agtgtcaaat agtgtatagt
2281 ggttgcacct acatgcttga gtctgatggc aagggcaatg tgaaagttga caggatccaa
2341 agcgccgccg tgcagaccaa caatgagctg gctggtgccc tgcaccactt aaggtgtgcc
2401 agaataagat actatgtcaa gtgtgtccag gaagccctgt attccatcat ccaaattgct
2461 ggggctgcat ttgtcaccac gcgcattgcc aagcgcatga acatacaaga cctatggtcc
2521 aagccacaag tggaaaacac agaggagacc accagcaagg acgggtgccc aaaacctaag
2581 gatgatgagg agtttgtcat ttcatccgac gacatcaaaa ctgagggcaa gaaagggaag
2641 aacaagactg gccgtggcaa gaagcacaca gcattttcaa gtaaaggcct cagtgatgaa
2701 gagtacgatg aatacaagag gattagagaa gaaagaaatg gcaagtactc tatagaagag
2761 taccttcagg acagggacaa atactatgag gaggtggcca ttgccagggc gactgaggaa
2821 gacttctgtg aagaggagga ggccaagatc cggcaaagga tctttaggcc aacaaggaaa
2881 caacgcaagg aggaaagagc ctccctcggt ttggtcacag gctctgaaat taggaaaaga
2941 aacccagatg acttcaaacc caaggggaaa ttgtgggctg acgatgacag gagtgtggac
3001 tacaatgaga aactcagttt tgaggcccca ccaagcattt ggtcgagaat agtcaacttt
3061 ggttcaggct ggggattctg ggtctccccc agtctgttca taacatcaac ccatgttata
3121 ccccagggcg caaaggagtt ctttggagtc cccatcaaac aaatacaggt acacaagtca
3181 ggcgagttct gtcgcttgag attccctaaa ccaatcagga ctgatgtgac gggcatgatc
3241 ttagaagaag gcgcacctga gggcaccgtg gtcacactac tcatcaaaag gcccactggg
3301 gaactcatgc ccctagcagc taggatgggg acccatgcga ccatgaagat ccaagggcgc
3361 actgttgggg gccagatggg catgcttctg acaggatcca acgccaagag tatggacctg
3421 ggtactacac caggtgattg tggctgcccc tatatctaca agagaggtaa tgactatgtg
3481 gtcattggag tccacacggc tgccgcacgt ggggggaaca ctgtcatatg tgccacccag
3541 gggagtgaag gagaggctac acttgagggt ggtgacaaca agggaacata ctgtggtgca
3601 ccaatcctag gcccagggag tgccccaaca cttagcacca agaccaaatt ctggagatcg
3661 tccacagcat cactcccacc tggcacctat gaaccagcct atcttggtgg caaggaccct
3721 agagtcaagg gtggcccttc actgcagcaa gtcatgaggg aacagttgaa gccattcaca
3781 gagcccaggg gcaagccacc aaaaccaagt gtattagaag ctgccaagaa gaccatcatc
3841 aatgtccttg agcaaacaat tgatccacct gagaagtggt cgttcgcaca agcttgcgcg
3901 tcccttgaca agaccacttc cagtggtcat ccgcatcaca tgcggaaaaa cgactgctgg
3961 aatggggagt ccttcacagg caagctggca gaccaggctt ccaaggccaa cctgatgttt
4021 gaagaaggga agaacatgac cccagtctac acagctgcgc tcaaggatga gttagttaaa
4081 actgacaaaa tttatggtaa gatcaagaag aggcttcttt ggggctcgga cttggcgacc
4141 atgatccggt gtgctcgagc attcggaggc ctaatggatg aactcaaagc gcactgtgtc
4201 acactcccca ttagagttgg catgaatatg aatgaggatg gccccatcat cttcgagagg
4261 cattccaggt acacgtacca ctatgatgct gattactctc ggtgggattc aacacaacag
4321 agagccgtat tggcagcagc tctagaaatc atggttaaat tctccccaga accacaattg
4381 gctcaggtag ttgcggaaga ccttctttct cctagcgtgg tggacgtggg cgacttcaca
4441 atatcaatca atgagggtct tccctctggg gtgccctgca cctcccaatg gaactccatc
4501 gcccactggc ttctcactct ctgtgcgctc tctgaagtca caaacctgtc tcctgacacc
4561 atacaggcta attccctctt ctctttttat ggtgatgatg aaattgttag cacagacata
4621 aaattggacc cagagaaatt gacaacaaag ctcagagaat atgggttaaa accaacccgc
4681 cctgacaaaa ctgaaggacc ccttgtcatc tctgaagacc tgaatggcct aacttttctg
4741 cggagaactg tgacccgcga cccagctggt tggtttggaa aactggagca gagttcaata
4801 ctcaggcaaa tgtactggac taggggtccc aaccacggag acccatctga aacaatgatc
4861 ccacactccc aaagacccat acaattgatg tccctactgg gggaggccgc tctccacggc
4921 ccagcatttt acagtaaaat cagcaaattg gtcattgcag agcttaaaga aggtggtatg
4981 gatttttacg tgcccagaca agagcctatg ttcagatgga tgagattctc agatctgagc
5041 acgtgggagg gcgatcgcaa tctggctccc agttttgtga atgaagatgg cgtcgagtga
5101 cgccaaccca tctgatgggt ccgcagccaa cctcgtccca gaggtcaaca atgaggttat
5161 ggctttggag cccgttgtcg gtgccgctat tgcggcgcct gtagcgggcc aacaaaatgt
5221 aattgacccc tggattagaa gtaactttgt acaagcccct ggtggagagt tcacagtgtc
5281 ccctagaaac gctccaggtg aaatactatg gagcgcgccc ttaggccctg atctgaatcc
5341 ctacctatct catttggcca gaatgtataa tggttatgca ggtggttttg aagtgcaggt
5401 gatcctcgcg gggaacgcgt tcaccgcggg aaaaatcata tttgcagcag ttccaccaaa
5461 ttttccaact gaaggcttga gtcccagcca agttactatg ttcccccaca taatagtaga
5521 tgttaggcaa ttggaacctg tgttgatccc cttacctgat gttaggaata acttctatca
5581 ctataaccag tcaaatgatt ctaccattaa attgatagca atgctgtaca caccactcag
5641 agccaataat gccggggatg atgtcttcac ggtctcttgt cgagtcctca cgaggccatc
5701 ccctgatttt gacttcatat ttctggtgcc acctacagtt gagtcaagaa ctaagccatt
5761 tactgtccca atcttgactg ttgaagaaat gaccaattca agattcccca ttcctttgga
5821 gaaattgttc acgggtccca gcagtgcctt tgttgttcaa ccacaaaatg gcagatgcac
5881 aactgatggc gtgctcttag gcaccaccca actgtctcct gtcaacatct gcaccttcag
5941 aggggatgtc acccacattg cgggttcccg taattacaca atgaatttgg cctctctaaa
6001 ttggagcaat tacgacccaa cagaagagat tccagcccct ctgggaactc cagatttcgt
6061 gggaaagatc caaggtgtgc tcactcaaac cacaaaggga gatggctcga cccggggcca
6121 taaagctaca gtttacactg ggagtgccga cttcactcca aagctgggca gtattcaatt
6181 tgctactgat acagaaaatg actttgaaac tcaccaaaac acaagattca ccccagtcgg
6241 tgtcattcag gatggtggca ccacccaccg aaatgaaccc caacaatggg tgctcccaag
6301 ttactcaggt agaaatgtcc ataatgtaca cctagcccct gctgtagccc ccaattttcc
6361 aggtgaacag ctccttttct tcaggtccac tatgcccgga tgtagcggat atcccaacat
6421 ggatttggat tgcctactcc cccaggagtg ggtgcaacac ttttaccaag aggcagctcc
6481 agcacaatct gatgtggctc tattgagatt tgtgaatcca gacacgggta gggtcttgtt
6541 tgagtgcaaa ctccataaat caggctatgt cacagtggct cataccggcc aacatgattt
6601 ggtcattccc cccaatggtt attttaggtt tgattcctgg gttaatcagt tctacacact
6661 tgcccccatg ggaaatggaa cggggcgtag acgtgcttta taatggctgg agctttcttt
6721 gctggattgg catctgatgt ccttagctct ggacttggtt ccctaatcaa tgctggggct
6781 ggggctatca accaaaggat tgattttgaa aataatagaa aattgcagca agcttccttt
6841 cagtttagta gtaatctaca acaagcttcc tttcaacatg ataaagagat gctccaagca
6901 caaattgagg ctactaaaaa gttgcaacag gaactgatga aagtcaaaca ggcaatgctc
6961 ttagaaggtg gattctctga aacagatgca gcccgtgggg caatcaacgc ccccatgaca
7021 aaggctttgg actggagtgg aacaaggtac tgggcccctg atgctaggac tacaacatac
7081 aatgcaggcc gcttttctac ccctcaacct tcgggggcac tgccaggaag aatcaacccc
7141 aggactccta tccccgcccg gggctcccca agcatatctt ccaatgtttc tactgctaat
7201 tctatacatt caaatcaaac tgcttcaacg agacttggtt ctacagctgg ttctggcacc
7261 gatgtctcga gtctcccgtc aactgcaagg actaggagtt gggttgagga tcaaaacaga
7321 aatttatcac ctttcatgag gggggctcac aacatatcgt ttgtcacccc accatctagc
7381 agatcctcca gccaaggcac agtctcaacc gtgcctaaag atgttttgga ctcctggact
7441 ggcgctttca acacgcgcag gcagcctctc ttcgctcaca ttcgtaggcg aggggagtca
7501 cgggtgtaa
//