Typing tool
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Typing tool
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Complete norovirus genomes
| KC409243 | GII.4 New Orleans | |
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| GII.P4 New Orleans |
ORF1: 1..5100
ORF2: 5081..6703
ORF3: 6703..7509
LOCUS KC409243 7509 bp ss-RNA linear VRL 13-MAR-2013
DEFINITION Norovirus Hu/GII/10411/2010/VNM, complete genome.
ACCESSION KC409243
VERSION KC409243.1
DBLINK BioProject: PRJNA70471
KEYWORDS .
SOURCE Norovirus Hu/GII/10411/2010/VNM
ORGANISM Norovirus Hu/GII/10411/2010/VNM
Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
Picornavirales; Caliciviridae; Norovirus; Norwalk virus.
REFERENCE 1 (bases 1 to 7509)
AUTHORS Madupu,R., Halpin,R.A., Ransier,A., Fedorova,N., Tsitrin,T.,
McLellan,M., Stockwell,T., Amedeo,P., Appalla,L., Bishop,B.,
Edworthy,P., Gupta,N., Hoover,J., Katzel,D., Li,K., Schobel,S.,
Shrivastava,S., Thovarai,V., Wang,S., My,P.V., Campbell,J.,
Farrar,J., Vinh,H., Hoang,N.V., Wentworth,D.E. and Baker,S.
TITLE Direct Submission
JOURNAL Submitted (17-DEC-2012) J. Craig Venter Institute, 9704 Medical
Center Drive, Rockville, MD 20850, USA
COMMENT This work was supported by the National Institute of Allergy and
Infectious Diseases (NIAID), Genome Sequencing Centers for
Infectious Diseases (GSCID) program.
The genome sequence was generated using overlapping PCR amplicons
spanning the genome. The amplicons were pooled by sample and then
barcoded and sequenced using Next Generation Sequencing platforms.
The consensus sequences of the internal PCR primer hybridization
sites were manually verified using reads from amplicons that
spanned across the sites.
Genome sequence lacks part of non-coding region.
##Genome-Assembly-Data-START##
Current Finishing Status :: Finished
Assembly Method :: clc_ref_assemble_long v. 3.22.55705
Genome Coverage :: 259.0x
Sequencing Technology :: Sanger; Illumina; 454
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7509
/organism="Norovirus Hu/GII/10411/2010/VNM"
/mol_type="genomic RNA"
/strain="Hu/GII/10411/2010/VNM"
/host="Homo sapiens; sex: F"
/db_xref="taxon:1291814"
/country="Viet Nam: Ho Chi Minh City"
/collection_date="19-Apr-2010"
/PCR_primers="fwd_name: Ampl_1_Forward, fwd_seq:
gtgaatgawgatggcgt, rev_name: Ampl_1_Reverse, rev_seq:
ttggttgagagyttyctg"
/PCR_primers="fwd_name: Ampl_2_Forward, fwd_seq:
ccgcaaaatcttcaagtg, rev_name: Ampl_2_Reverse, rev_seq:
cwacaggtcttggtctgctrga"
/PCR_primers="fwd_name: Ampl_3_Forward, fwd_seq:
tcaaccaartctgcttcacctg, rev_name: Ampl_3_Reverse, rev_seq:
ratcctttgccggatcttgg"
/PCR_primers="fwd_name: Ampl_4_Forward, fwd_seq:
ggcaagaagcacacagcctt, rev_name: Ampl_4_Reverse, rev_seq:
crrctctytgttgtgttgaatccc"
/PCR_primers="fwd_name: Ampl_5_Forward, fwd_seq:
tggyaagatcaagaagaggc, rev_name: Ampl_5_Reverse, rev_seq:
acaacaaargcacygctrgg"
/PCR_primers="fwd_name: Ampl_6_Forward, fwd_seq:
gagrccrtccccygattttg, rev_name: Ampl_6_Reverse, rev_seq:
atcaatyttgtcttttcaca"
/note="genotype: II"
gene 1..5100
/gene="POL"
CDS 1..5100
/gene="POL"
/note="genome polyprotein"
/codon_start=1
/product="nonstructural polyprotein"
/protein_id="AGE89447.1"
/translation="MKMASNDASAAAVANSNNDTAKSSSDGVLSSMAVTFKRALGARP
KQPPPREKPQRPPRPPTPELVKNIPPPPPNGEDEIVVSYSVKDGVSGLPDLSTVRQPE
ESNTAFSVPPLNQRENRDAKEPLTGTILEMWDGEIYHYGLYVERGLVLGVHKPPAAIS
LARVELAPLSLYWRPVYTPQYLISPDTLKKLSGETFPYTAFDNNCYAFCCWVLDLNDS
WLSRRMIQRTTGFFRPYQDWNRKPLPTMDDSKIKKVANIFLCALSSLFTRPIKDIIGK
IRPLNILNILASCDWTFAGIVESLILLAELFGVFWTPPDVSAMIAPLLGDYELQGPED
LAVELVPVVMGGIGLVLGFTKEKIGKMLSSAASTLRACKDLGAYGLEILKLVMKWFFP
KKEEANELAIVRSIEDAVLDLEAIENNHMTTLLKDKDSLATYMRTLDLEEEKARKLST
KSASPDIVGTINALLARIAAARSLVHRAKEELSSRPRPVVLMISGRPGIGKTHLAREV
AKRIAASLTGDQRVGLIPRNGVDHWDAYKGERVVLWDDYGMSNPIHDALRLQELADTC
PLTLNCDRIENKGKVFDSDVIIITTNLANPAPLDYVNFEACSRRIDFLVYAEAPEVEK
AKRDFPGQPDMWKNAFSSDFSHIKLALAPQGGFDKNGNTPHGKGVMKTLTTGSLIARA
SGLLHERLDEFELQGPALTTFNFDRNKVLAFRQLAAENKYGLMDTMRVGKQLKDVRTM
PELKQALKNVSIKKCQIVYSGCTYILESDGKGNVKVDRIQSAAVQTNNELAGALHHLR
CARIRYYVKCVQEALYSIIQIAGAAFVTTRIAKRMNIQDLWSKPQVENTEETTSKDGC
PKPKDDEEFVISSDDIKTEGKKGKNKTGRGKKHTAFSSKGLSDEEYDEYKRIREERNG
KYSIEEYLQDRDKYYEEVAIARATEEDFCEEEEAKIRQRIFRPTRKQRKEERASLGLV
TGSEIRKRNPDDFKPKGKLWADDDRSVDYNEKLSFEAPPSIWSRIVNFGSGWGFWVSP
SLFITSTHVIPQGAKEFFGVPIKQIQVHKSGEFCRLRFPKPIRTDVTGMILEEGAPEG
TVVTLLIKRPTGELMPLAARMGTHATMKIQGRTVGGQMGMLLTGSNAKSMDLGTTPGD
CGCPYIYKRGNDYVVIGVHTAAARGGNTVICATQGSEGEATLEGGDNKGTYCGAPILG
PGSAPTLSTKTKFWRSSTASLPPGTYEPAYLGGKDPRVKGGPSLQQVMREQLKPFTEP
RGKPPKPSVLEAAKKTIINVLEQTIDPPEKWSFAQACASLDKTTSSGHPHHMRKNDCW
NGESFTGKLADQASKANLMFEEGKNMTPVYTAALKDELVKTDKIYGKIKKRLLWGSDL
ATMIRCARAFGGLMDELKAHCVTLPIRVGMNMNEDGPIIFERHSRYTYHYDADYSRWD
STQQRAVLAAALEIMVKFSPEPHLAQVVAEDLLSPSVVDVGDFTISINEGLPSGVPCT
SQWNSIAHWLLTLCALSEVTNLSPDTIQANSLFSFYGDDEIVSTDIKLDPEKLTAKLR
EYGLKPTRPDKTEGPLVISEDLNGLTFLRRTVTRDPAGWFGKLEQSSILRQMYWTRGP
NHGDPSETMIPHSQRPIQLMSLLGEAALHGPAFYSKISKLVIAELKEGGMDFYVPRQE
PMFRWMRFSDLSTWEGDRNLAPSFVNEDGVE"
mat_peptide 1..990
/gene="POL"
/product="protein p48"
mat_peptide 991..2088
/gene="POL"
/product="NTPase"
/note="p41"
mat_peptide 2089..2625
/gene="POL"
/product="protein p22"
mat_peptide 2626..3024
/gene="POL"
/product="viral genome-linked protein"
/note="VPg"
mat_peptide 3025..3567
/gene="POL"
/product="3C-like protease"
/note="3CLpro; calcivirin"
mat_peptide 3568..5097
/gene="POL"
/product="RNA-directed RNA polymerase"
gene 5081..6703
/gene="VP1"
CDS 5081..6703
/gene="VP1"
/codon_start=1
/product="capsid protein VP1"
/protein_id="AGE89448.1"
/translation="MKMASSDANPSDGSTANLVPEVNNEVMALEPVVGAAIAAPVAGQ
QNVIDPWIRNNFVQAPGGEFTVSPRNAPGEILWSAPLGPDLNPYLSHLARMYNGYAGG
FEVQVILAGNAFTAGKIIFAAVPPNFPTEGLSPSQVTMFPHIIVDVRQLEPVLIPLPD
VRNNFYHYNQSNDPTIKLIAMLYTPLRANNAGDDVFTVSCRVLTRPSPDFDFIFLVPP
TVESRTKPFSVPVLTVEEMTNSRFPIPLEKLFTGPSSAFVVQPQNGRCTTDGVLLGTT
QLSPVNICTFRGDVTHIPGSRNYTMNLASQNWNSYDPTEEIPAPLGTPDFVGKIQGVL
TQTTRTNGSTRGHKATVYTGSADFSPKLGRVQFATDTDNDFETNQNTKFTPVGVIQDG
STTPRNEPQQWVLPSYSGRNIHNVHLAPAVAPTFPGEQLLFFRSTMPGCSGYPNMDLD
CLLPQEWVQYFYQEAAPAQSDVALLRFVNPDTGRVLFECKLHKSGYVTVAHTGQHDLV
IPPNGYFRFDSWVNQFYTLAPMGNGTGRRRAL"
gene 6703..7509
/gene="VP2"
CDS 6703..7509
/gene="VP2"
/note="minor capsid protein"
/codon_start=1
/product="capsid protein VP2"
/protein_id="AGE89449.1"
/translation="MAGAFFAGLASDVLGSGLGSLINAGAGAINQKVEFENNRKLQQA
SFQFSSNLQQASFQHDKEMLQAQIEATKKLQQEMMRVKQAMLLEGGFSETDAARGAIN
APMTKTLDWSGTRYWAPDARTTTYNAGRFSTPQPSGVLPGRANLRATVPARGSSSTFS
NSSIATSVYSNQTTSTRLGSTAGSGTSVSSLPSTARTRSWVEDQNRNLSPFMRGAHNI
SFVTPPSSRSSSQGTVSTVPKEVLDSWTGAFNTRRQPLFAHIRKRGESRV"
ORIGIN
1 atgaagatgg cgtctaacga cgcttccgct gccgctgttg ctaacagcaa caacgacacc
61 gcaaaatctt caagtgacgg agtgctttct agcatggctg tcacttttaa acgagccctc
121 ggggcgcggc ctaaacagcc tcccccgagg gaaaaaccac aaagaccccc acgaccacct
181 actccagaac tggttaaaaa tattccccct cccccaccca acggagagga tgaaatagtg
241 gtttcttata gtgtcaaaga tggtgtttcc ggcttgcctg acctttccac cgtcaggcaa
301 ccggaagaat ctaacacggc cttcagtgtc cctccactca atcagaggga gaatagagat
361 gctaaggaac cacttactgg aacaattctg gaaatgtggg acggggaaat ctaccattat
421 ggcctgtatg tggagcgagg tcttgtacta ggtgtgcaca aaccaccagc tgccatcagc
481 ctcgctaggg ttgagctagc accactctcc ttgtactgga gacctgtgta cactcctcag
541 tacctcatct ctccagacac tctcaagaaa ttatccggag aaacgttccc ctacacagcc
601 tttgacaaca actgttatgc cttttgttgc tgggtcctgg acctaaatga ctcgtggctg
661 agcaggagaa tgatccagag aacaactggt ttcttcaggc cctaccaaga ctggaatagg
721 aaaccccttc ccactatgga tgactccaaa ataaagaagg tagctaacat attcctgtgt
781 gctctgtcct cgctgttcac cagacccata aaagatataa tagggaagat aaggcctctt
841 aacatcctca acatcttagc ctcatgtgat tggacttttg caggtatagt ggagtccctg
901 atactcttgg cagaactctt tggagttttc tggacacccc cagatgtgtc tgcgatgatt
961 gcccccttac ttggtgacta cgagctacaa ggacctgagg accttgcagt tgagctcgtc
1021 cccgtggtga tggggggaat tggtttggtg ctaggattca ccaaagagaa gattgggaaa
1081 atgttgtcat ctgctgcgtc taccttgaga gcttgtaaag accttggtgc atatgggcta
1141 gagatcctaa agttggtcat gaagtggttc ttcccgaaga aggaagaggc aaatgagctg
1201 gctatagtga ggtctatcga ggatgcagtc ctggacctcg aggcaattga aaacaaccat
1261 atgaccacct tgctcaaaga caaagacagt ctggcaacct atatgagaac acttgacctt
1321 gaggaggaga aagccaggaa actctcaacc aagtctgcct cacccgacat cgtgggcaca
1381 atcaacgccc tcctggcgag aatcgctgcc gcacgttctc tggtgcaccg agcgaaggag
1441 gagctttcca gcagaccaag acctgtggtg ttgatgatat caggcaggcc aggaataggg
1501 aagacccacc tcgctaggga agtggctaag agaatcgcag cctcccttac aggagaccag
1561 cgtgtgggcc tcatcccacg caatggcgtc gaccattggg atgcgtacaa gggggagagg
1621 gtcgtcctat gggacgatta tggaatgagc aaccctattc acgatgccct caggctgcaa
1681 gaactcgctg acacttgccc cctcactcta aactgtgaca ggatcgaaaa taaaggaaag
1741 gtctttgaca gcgatgtcat cattatcacc actaatctgg ccaacccagc gccactggac
1801 tatgtcaact ttgaagcatg ctcgaggcgc atcgacttcc tcgtgtatgc agaagcccct
1861 gaagtcgaaa aggcgaagcg tgacttccca ggccagcctg acatgtggaa gaacgccttc
1921 agttctgatt tctcacacat aaaactagca ctggccccac agggtggttt cgacaagaac
1981 gggaacaccc cacacggaaa gggcgttatg aagactctca ccactggctc ccttattgcc
2041 cgggcatcag ggttactcca tgagaggtta gatgaatttg aactgcaggg cccagctctc
2101 accaccttca atttcgatcg caataaggtg cttgccttta gacagcttgc tgctgaaaat
2161 aaatatggat tgatggacac aatgagagtt gggaaacagc tcaaggatgt cagaaccatg
2221 ccagaactca aacaagcact caagaatgtc tcaatcaaga agtgccaaat agtgtatagt
2281 ggttgcacct acatacttga gtctgatggc aagggcaatg tgaaagttga cagaatccaa
2341 agcgccgccg tgcagaccaa caatgagctg gctggtgccc tgcaccattt gaggtgcgcc
2401 agaatcagat actatgtcaa gtgtgtccag gaggccctgt attccatcat tcaaattgct
2461 ggggctgcat ttgtcaccac gcgcattgcc aagcgcatga acatacaaga cctatggtcc
2521 aagccacaag tggaaaacac agaggagact accagcaagg acgggtgccc aaaacctaag
2581 gacgatgagg agtttgtcat ttcatccgac gacatcaaaa ctgagggtaa gaaagggaag
2641 aacaagactg gccgcggcaa gaagcacaca gcattttcaa gcaaaggcct cagtgatgaa
2701 gagtacgatg agtacaagag gatcagagaa gaaaggaacg gcaagtactc catagaagag
2761 taccttcagg acagggacaa atactatgag gaggtggcca ttgccagggc gactgaggaa
2821 gacttctgtg aagaggagga ggccaagatc cggcaaagga tctttaggcc aacaaggaaa
2881 caacgcaagg aggaaagagc ctctctcggt ctggtcacag gctctgaaat taggaaaaga
2941 aacccagatg acttcaaacc caaagggaaa ttgtgggctg acgatgacag gagtgtggac
3001 tacaatgaga aactcagttt tgaggcccca ccaagcatct ggtcgagaat agtcaacttt
3061 ggttcaggct ggggattttg ggtctccccc agtctgttca taacatcaac ccatgtcata
3121 ccccagggcg caaaggagtt ctttggagtc cccatcaaac aaatacaggt acacaagtca
3181 ggcgagttct gtcgcttgag attcccaaaa ccaatcagga ctgatgtgac gggcatgatc
3241 ttagaagaag gcgcacctga gggcactgtg gtcacactac tcatcaaaag gcccactggg
3301 gaactcatgc ccctagcagc taggatgggt acccatgcga ccatgaagat ccaagggcgc
3361 actgttggag gccagatggg catgcttctg acaggatcca acgccaagag catggatctg
3421 ggtactacac caggtgattg tggctgcccc tatatctaca agagaggtaa tgactatgtg
3481 gtcattgggg tccacacggc tgccgcacgt ggggggaaca ctgtcatatg tgccacccag
3541 gggagtgaag gagaggctac acttgaaggt ggtgacaaca aggggacata ctgtggtgca
3601 ccaatcctag gcccagggag tgccccaaca cttagcacca agaccaaatt ctggagatcg
3661 tccacagcat cactcccacc tggcacctat gaaccagcct atcttggtgg caaggaccct
3721 agggtcaagg gtggcccttc actgcagcaa gtcatgaggg agcagttgaa gccattcaca
3781 gagcccaggg gcaagccacc aaaaccaagt gtattagaag ctgccaagaa gaccatcatc
3841 aatgtccttg agcaaacaat tgatccacct gagaagtggt cgttcgcaca agcctgcgcg
3901 tcccttgaca agaccacttc cagtggtcat ccgcaccaca tgcggaaaaa cgactgctgg
3961 aacggggagt ccttcacagg caagctggca gaccaggctt ccaaggccaa cctgatgttt
4021 gaagaaggga agaacatgac cccagtctac acagctgcgc tcaaggatga gttagttaaa
4081 actgacaaaa tttatggtaa gatcaagaag aggcttctct ggggctcgga cttggcgacc
4141 atgatccggt gtgctcgagc attcggaggc ctaatggatg aactcaaagc acactgtgtc
4201 acacttccca ttagagttgg catgaatatg aatgaggatg gccccatcat cttcgagaga
4261 cattccaggt acacgtatca ctatgatgct gattactctc gatgggattc aacacaacag
4321 agagccgtgt tggcagcagc tctagaaatc atggttaaat tctccccaga accacacttg
4381 gctcaggtag tcgcggagga ccttctttct cctagcgtgg tggacgtggg cgacttcaca
4441 atatcaatca acgagggtct tccctctggg gtgccctgca cctcccaatg gaactccatc
4501 gcccactggc ttctcactct ctgtgcgctc tctgaagtca caaacctgtc ccctgatacc
4561 atacaggcta actccctctt ctctttttat ggtgatgatg aaattgttag cacagacata
4621 aaattggacc cagaaaaatt gacagcaaag ctcagagaat atgggttaaa accaacccgc
4681 cctgacaaaa ctgaaggacc ccttgtcatc tctgaagacc tgaatggcct aactttcctg
4741 cggagaactg tgacccgcga cccagctggt tggtttggaa aactggagca gagttcaata
4801 ctcaggcaaa tgtactggac taggggtccc aaccatggag acccatctga aacaatgatt
4861 ccacactccc aaagacccat acaattgatg tccctactgg gggaggccgc tctccacggc
4921 ccagcatttt acagcaaaat cagcaaattg gtcattgcag agctaaaaga aggtggcatg
4981 gatttttacg tgcccagaca agagccaatg ttcagatgga tgagattctc agatctgagc
5041 acgtgggagg gcgatcgcaa tctggctccc agttttgtga atgaagatgg cgtcgagtga
5101 cgccaaccca tctgatgggt ccacagccaa ccttgtccca gaggtcaata atgaggttat
5161 ggctttggag cccgtagttg gtgccgccat tgcggcacct gtagcgggcc aacaaaatgt
5221 aattgacccc tggattagaa acaattttgt acaagcccct ggtggagagt tcacagtatc
5281 ccctagaaac gctccaggtg aaatactatg gagcgcgccc ttaggccctg atttgaatcc
5341 ctacctttcc catttggcca gaatgtacaa tggttatgca ggtggttttg aagtgcaagt
5401 aatcctcgcg gggaacgcgt tcaccgccgg gaaaatcata tttgcagcag tcccaccaaa
5461 tttcccaact gaaggtttga gccccagcca ggtcactatg ttcccccaca taatagtaga
5521 tgttaggcaa ttggaacctg tgttgattcc cttacccgat gttaggaata atttctacca
5581 ttataatcaa tcaaatgacc ccaccatcaa attgatagca atgttgtaca caccacttag
5641 ggctaataat gccggggacg atgtcttcac agtttcttgt cgagttctca cgagaccatc
5701 ccccgatttt gatttcatat ttttggtgcc acccacagtt gaatcaagaa ctaaaccatt
5761 ctctgtccca gttttaactg ttgaggagat gaccaattca aggttcccca ttcctttgga
5821 aaagttgttc acgggcccca gtagtgcctt tgttgttcaa ccacaaaacg gcaggtgcac
5881 gactgatggc gtgctcctag gtactaccca actgtctccc gtcaacatct gcaccttcag
5941 aggggacgtc acccatattc caggcagtcg taactacaca atgaatttgg cctcccaaaa
6001 ttggaacagt tacgatccaa cagaagaaat cccagcccct ctaggaactc cagatttcgt
6061 ggggaagatt caaggtgtgc tcacccaaac cacaaggaca aatggctcga cccgcggcca
6121 caaagctaca gtgtacactg ggagcgccga cttttctcca aaactgggta gagttcaatt
6181 tgccactgac acagacaatg attttgaaac taaccaaaac acaaagttca ccccagtcgg
6241 tgttatccag gatggtagta ccaccccccg aaatgaaccc caacaatggg tgctcccaag
6301 ttactcaggc agaaacattc ataatgtgca cctggccccc gctgtagccc ctactttccc
6361 gggcgagcag ctcctcttct tcagatctac tatgcccgga tgcagcgggt accccaacat
6421 ggacttggac tgtctgctcc cccaggaatg ggtgcaatat ttctaccagg aggcagcccc
6481 agcacaatct gatgtggctc tgctaagatt tgtgaatcct gacacaggta gggttttgtt
6541 tgagtgtaag cttcataaat caggctatgt tacagtggct cacactggcc aacatgattt
6601 ggttatcccc cccaatggtt attttagatt tgattcctgg gtcaaccagt tctacacact
6661 tgcccccatg ggaaatggga cggggcgtag acgtgcatta taatggctgg agctttcttt
6721 gctggattgg catctgacgt ccttggctct ggacttggtt ccctaatcaa tgctggggct
6781 ggggccatca atcaaaaagt tgaatttgaa aataacagaa aattgcaaca agcttccttc
6841 caatttagta gcaatctaca acaggcttcc tttcaacatg acaaagagat gctccaagca
6901 caaattgagg ccaccaaaaa gttgcaacag gaaatgatga gagttaaaca agcaatgctt
6961 ctagagggtg gattctctga gacagatgca gcccgtgggg caatcaacgc ccccatgaca
7021 aaaactttgg actggagcgg gacaaggtac tgggctcccg atgctaggac tacaacatat
7081 aatgcaggcc gcttttccac cccccaaccc tcgggggtac taccaggaag agctaatctt
7141 agggctactg tccccgcccg gggttcctcc agcacgttct ctaactcttc tattgctact
7201 tctgtgtatt caaatcaaac cacctcaacg agacttggtt ctacagctgg ttctggtacc
7261 agtgtctcga gcctcccgtc aactgcaagg actaggagct gggttgagga tcaaaatagg
7321 aatttgtcac ctttcatgag gggggcccat aacatctcgt ttgtcacccc accatctagc
7381 agatcctcta gccaaggcac agtctcaacc gtgcccaaag aagttttgga ctcctggact
7441 ggcgctttca acacgcgcag gcagcctctc ttcgctcaca ttcgcaagcg aggggagtca
7501 cgggtgtaa
//